North Carolina State University

Duke University

Chase Beisel

Charles A Gersbach

Adrian Pickar Oliver

Michelle Luo

Ahmed M Gomaa

Ahmed Abdelshafy Mahmoud Gomaa

Disclosed herein are Type I Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas) system related compositions and methods of using said Type I CRISPR/Cas system related compositions for altering gene expression and genome engineering. The invention relates to compositions comprising Type I CRISPR-Cas polypeptides and CRISPR array nucleic acids designed for genome modification in eukaryotic cells and for targeted killing of eukaryotic cells.

Genome engineering with Type I CRISPR systems in eukaryotic cells

The present invention is directed to methods and compositions for targeted gene silencing that provide the ability to not only repress expression but to modulate the repression of expression of one or more target genes. In one aspect, a recombinant nucleic acid molecule is provided comprising a nucleotide sequence encoding a subset of CRISPR-cas polypeptides, or functional fragments thereof, from a type-I CRISPR-cas system. In some aspects, a recombinant nucleic acid of the invention comprises a nucleotide sequence encoding three or more Type I Cascade polypeptides having substantial identity to a type I Cascade polypeptide.

Methods and compositions for RNA-directed repression of transcription using CRISPR-associated genes

Genome engineering with type I CRISPR systems in eukaryotic cells

The invention relates to antimicrobial compositions comprising cell-penetrating peptides linked to CRISPR RNAs and methods for their use.

Methods and compositions for sequence specific antimicrobials

The invention relates to the methods for modifying the methylation pattern of bacteriophage DNA and phagemid DNA and to methods for selective killing of bacteria using lysogenic bacteriophages comprising bacteriophage DNA or phagemid DNA comprising components of an engineered CRISPR-Cas system.

Growing community of inventors

Raleigh, NC, United States of America

Chase Beisel