Fraunhofer-Gesellschaft Zur Foerderung Der Angewandten Forschung E.V.

Charli Kruse

Guenter R Fuhr

Gunter Fuhr

Philipp Ciba

Heiko Zimmermann

Günter R Fuhr

Daniel Hans Rapoport

Hagen Von Briesen

Robert Lemor

Sandra Danner

Thilo Wedel

Bianka Grunow

Erwin Gorjup

Norbert W Guldner

Jennifer Kajahn

Günter Fuhr

Tim Becker

Daniel Rapoport

Emel GÜRLEYIK

Lars Lüllwitz

Marina Gebert

Jürgen Rohwedel

The invention relates to methods for producing three-dimensional multicellular tissue and organ culture systems using multicellular mammalian cell aggregates, these systems themselves and their use for various medical purposes, in particular implantations.

Multicellular tissue and organ culture systems

This invention relates to isolated pluripotent adult stem cells which are obtained from exocrine glandular tissue as well as methods of isolating and culturing these pluripotent stem cells.

Isolated adult pluripotent stem cells and methods for isolating and cultivating thereof

The invention relates to material compositions comprising adult stem cells obtained from exocrine gland tissue and a supporting matrix having the shape of a thread structure and/or of a net. The supporting matrix preferably consists of a plastic material which is physiologically acceptable and degradable in the body. The material compositions of the invention are in particular suited for use in regenerative medicine, e.g. for regeneration of injured or damaged myocard tissue.

Material compositions which comprise adult stem cells obtained from exocrine glandular tissue, in particular for use in regenerative medicine

A targeted cultivation phase adjustment is provided in a process for the cultivation of cells in which biological cells are cultivated on at least one boundary surface between different, non-solid cultivation phases so that pre-determined cultivation conditions are given. A cultivation apparatus () for biological cells is also provided that includes a culture container () with different, non-solid cultivation phases () between which at least one boundary surface is formed.

Process and culturing unit for culturing biological cells

The invention relates to a method for conserving at least one biological deep-sea organism () comprising the steps of collecting the biological organism () in a deep-sea trapping device (); cooling the biological organism () with a cooling device (), wherein the biological organism () is put in a frozen condition; and moving the frozen biological organism (), preferably with the trapping device (), to a normal-pressure environment. The invention further relates to a conservation apparatus () for conserving at least one biological deep-sea organism ().

Method and apparatus for conserving deep-sea organisms

The invention relates to an in vitro method for producing spontaneously contracting fish cell aggregates, to the fish cell aggregates obtained thereby, and to the use thereof, in particular for testing biological active substances and pharmaceuticals. The in vitro method according to the invention for producing contracting fish cell aggregates comprises the following steps: a) mechanical comminution and/or partial enzymatic digestion of fish embryos or fish larvae; b) transfer of the comminuted and/or partially digested fish tissue into an enzyme-free medium and removing the supernatant by centrifuging to obtain a cell pellet; c) re-suspension of the cell pellet in a cell culture medium; d) culturing the cells, with the medium being changed at least once, until spontaneously contracting cell aggregates form.

Spontaneously contracting fish cell aggregates, use thereof and method for the production thereof

A method for forming a three-dimensional cell arrangement () of biological cells is disclosed, including the steps of preparation of the cell arrangement () on a flexible substrate () and deformation of the substrate (), wherein the deformation of the substrate () is brought about by an attractive force exerted by the cells () on the substrate (). A substrate (), is also disclosed, made from a flexible material and including a substrate surface () for adhesion of a cell arrangement () of biological cells, wherein the substrate surface () has a number of force attachment points arranged to exert an attraction force which may be transmitted from the cells to the substrate () and the substrate () has a flexibility such that the substrate () is deformable with the action of the attraction force.

Method and device for forming a three-dimensional arrangement of biological cells

The invention relates to a method for cultivating biologic cells (), wherein cells () are grown on a substrate () having a plurality of substrate openings (), and wherein cell aggregates () are formed, including groups of cells () that span the substrate openings (). A separation of the cell aggregates () from the substrate () by extracting the cell aggregates () from the substrate openings () can be provided. The invention further relates to a cell-cultivating device (), including a substrate () having a plurality of substrate openings () and cell aggregates () including groups of cells () that span the substrate openings (). The cell aggregates () are particularly used in high throughput tests with biologically active substances or in methods of tissue cultivation.

Method and device for forming biologic cell aggregates

Disclosed is a method for forming epithelial cells. Said method comprises the steps of aggregating stem cells from differentiated exocrine gland tissue to obtain an organoid body and differentiating at least one portion of the organoid body or a tissue body grown therefrom to obtain epithelial cells. Also disclosed is a cultivation device, particularly for forming differential epithelial cells.

Growing community of inventors

Herrnburg, Germany

Charli Kruse