Imclone Systems, Inc.

The University of North Carolina at Chapel Hill

Princeton University

Charles T Tackney

Dana M Fowlkes

Susan M Skelly

John N Snouwaert

Neil I Goldstein

Daniel J Hicklin

Herbert Weich

Harlan W Waksal

Jurgen Hoppe

Wolfram Eichner

The present invention provides a polypeptide that is non-toxic in E. coli. The disclosed polypeptide comprises at least one antigenic sequence present in P.IA of N. gonorrhoeae and at least one antigenic sequence present in P.IB of N. gonorrhoeae. Further, the disclosed polypeptide of the invention is fused to a carrier peptide.

Recombinant hybrid porin epitopes

Muteins of IL-6 and truncated IL-6 are prepared by recombinant DNA techniques. In the muteins, the cysteine residues that occur at positions, or at positions corresponding to positions, 45 and 51 of mature, native IL-6 have been replaced by other amino acids. The cysteine residues that occur at positions, or at positions corresponding to positions, 74 and 84 are retained. The molecule has biological activity that is at least comparable to that of native IL-6.

Method of making cysteine depleted IL-6 muteins

Methods and materials for producing hepatitis virus HBe antigenic proteins useful in immunoassays without the necessity of maintaining these proteins in denaturing environments are disclosed. Assay methods and materials utilizing these HBe proteins are also provided.

Stabilized hepatitis B e antigen suitable for immunoassays

Cysteine depleted IL-6 muteins

The present invention provides a method for production of recombinant PDGF-B in prokaryotic cells. Also provided are DNA constructs for fusion protein useful in the production of the biologically active product.

PDGF-B fusion protein, vectors, and host cells for use in production

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Brooklyn, NY, United States of America

Charles T Tackney