Yale University

Charles Meyer Radding

David C Ward

Gurucharan Reddy

Efim Ilya Golub

Thomas Haaf

Sherman M Weissman

Saul M Honigberg

Andrew A Welcher

Basil Rigas

Compositions and methods are provided for identifying and diagnosing individuals at risk for a disease state associated with aberrant Rad41 foci.

Methods and compositions utilizing Rad51

Compositions and methods are provided for identifying agents which bind to or modulate Rad51.

The invention relates to complexes of eukaryotic Rad52 protein and nucleic acids, and methods of using the complexes.

Compositions and methods using eukaryotic Rad52

A stable, single-stranded nucleoprotein filament adapted to complex specifically and stably with a target duplex DNA having a selected base sequence. The filament is composed of a single-stranded DNA probe having a region of homology with the target base sequence, and RecA protein bound stably to the DNA probe by adenosine 5'-(.gamma.-thio)triphosphate. The filament is useful in a novel system and method for enriching target duplex DNA which contains a region homologous to the probe sequence, for blocking selected restriction endonuclease sites in the target DNA, and in other DNA methodologies in which stable rapid triple-strand synaptic formation in duplex DNA can be exploited.

Growing community of inventors

Hamden, CT, United States of America

Charles Meyer Radding