George Mason University

Charles L Bailey

Victor Morozov

Nikolai N Vsevolodov

Adam Elliott

Melissa R Evanskey

Aarthi Narayanan

Ken Alibek

Disclosed herein are single nucleic acid constructs comprising a vector, wherein the vector comprises: a first nucleic acid sequence, wherein the first nucleic acid sequence comprises one or more antigenic sequences of interest, one or more spacer sequences and one or more hydrophobic anchor sequences, operably linked to a first transcriptional control element; a second nucleic acid sequence, wherein the second nucleic acid sequence comprises one or more pathogen-associated molecular pattern (PAMP) sequences operably linked to a second transcriptional control element; a third nucleic acid sequence, wherein the third nucleic acid sequence comprises one or more cell surface receptor binding sequences operably linked to a third transcriptional control element; and a selectable marker. Also described herein, are methods of making the single nucleic acid constructs and methods of administering the single nucleic acid constructs for the treatment or prevention of infections and cancer. Also described herein are cell lines and engineered vesicles made using the single nucleic acid constructs described herein.

Engineered vesicles comprising antigenic peptides and the uses thereof as modulators of immune responses

The invention involves developing and stabilizing cultivating droplets within a matrix of a porous medium. A cultivation medium may be selected, prepared and mixed with a surfactant. Where cells are desired to be cultured in droplets, cells may be added. The mixture may be converted into cultivating droplets. The cultivating droplets may be stabilized by introducing them to a porous medium. The porous medium may contain hydrophobic particles. Stabilized, cultivating droplets having one or more cells may form an aseptic microenvironment for the concentrated growth of cells.

Cell culture of micorrhizal fungus and arthrobacter histidinolovorans

The invention contemplates apparatuses for recognition of proteins and other biological molecules by imaging morphology, size and distribution of crystalline and amorphous dry residues in droplets (further referred to as 'crystallization patterns') containing predetermined amount of certain crystal-forming organic compounds (reporters) to which protein to be analyzed is added. Changes in the crystallization patterns of a number of amino-acids can be used as a 'signature' of a protein added. Also, changes in the crystallization patterns, as well as the character of such changes, can be used as recognition elements in analysis of protein molecules.

Apparatus for detecting and recognizing analytes based on their crystallization patterns

Analytes using an active assay may be detected by introducing an analyte solution containing a plurality of analytes to a lacquered membrane. The lacquered membrane may be a membrane having at least one surface treated with a layer of polymers. The lacquered membrane may be semi-permeable to nonanalytes. The layer of polymers may include cross-linked polymers. A plurality of probe molecules may be arrayed and immobilized on the lacquered membrane. An external force may be applied to the analyte solution to move the analytes towards the lacquered membrane. Movement may cause some or all of the analytes to bind to the lacquered membrane. In cases where probe molecules are presented, some or all of the analytes may bind to probe molecules. The direction of the external force may be reversed to remove unbound or weakly bound analytes. Bound analytes may be detected using known detection types.

Analyte detection using an active assay

The claimed invention describes methods and apparatuses for manufacturing nano-aerosols and nano-structured materials based on the neutralization of charged electrosprayed products with oppositely charged electrosprayed products. Electrosprayed products include molecular ions, nano-clusters and nano-fibers. Nano-aerosols can be generated when neutralization occurs in the gas phase. Neutralization of electrospan nano-fibers with molecular ions and charged nano-clusters may result in the formation of fibrous aerosols or free nano-mats. Nano-mats can also be produced on a suitable substrate, forming efficient nano-filters.

Electrospray neutralization process and apparatus for generation of nano-aerosol and nano-structured materials

The invention contemplates a method for recognition of proteins and other biological molecules by imaging morphology, size and distribution of crystalline and amorphous dry residues in droplets (further referred to as 'crystallization pattern') containing predetermined amount of certain crystal-forming organic compounds (reporters) to which protein to be analyzed is added. It has been shown that changes in the crystallization patterns of a number of amino-acids can be used as a 'signature' of a protein added. It was also found that both the character of changer in the crystallization patter and the fact of such changes can be used as recognition elements in analysis of protein molecules.

Detection and recognition of analytes based on their crystallization patterns

The present invention comprises a novel culture method and device in which living cells are cultured in a plurality of individual microdroplets that are immobilized and isolated within a matrix of hydrophobic particles. The hydrophobic particles adhere to inoculated microdroplets of media, isolating the microdroplets in an aseptic microenvironmet. The plurality of individual microdroplets provide and optimal environment for the concentrated growth of cultured cells contained therein.

Growing community of inventors

Cross Junction, VA, United States of America

Charles L Bailey