Ventana Medical Systems, Inc.

Charles H Weidner

Edward E Durrant

Casey A Kernag

Brian Howard Kram

Chad Wilkinson

Melinda Polaske

Heidi Barnett

Kristina Orosz

Zofia Wosinska

Daniel Champion

Henning Groll

Parula Mehta

Setareh Duquette

Kenneth S Weir

Cynthia Connolly

Lesley Schumack

Kenneth Weir

Methods and compositions for removing precipitates or reducing the formation of precipitates generated in hematoxylin solutions. The methods and compositions feature cleaning solutions that feature chemical compounds that initiate processes including but not limited to acidification of the waste solution, chelation of metal ions in the waste solution, reduction reactions, oxidation reactions, and metal salt addition reactions.

Methods and compositions for removing or reducing formation of precipitates generated in hematoxylin solutions

Processing specimens in an automated histological staining system comprising robotically moving a slide carrier into a stainer of the system, the slide carrier carrying slides which respectively carry the specimens, and the specimens being at least partially embedded in paraffin. Liquids are automatically dispensed onto the slides according to a predetermined recipe for at least deparaffinizing, staining, and counterstaining the specimens. The slide carrier can be robotically moved out of the stainer after automatically dispensing the liquids. In some embodiments, a total of all liquid dispensed onto the slides after moving the slide carrier into the stainer and before moving the slide carrier out of the stainer has a greater volumetric concentration of polyol than of monohydric alcohol.

Staining reagents and other liquids for histological processing of biological specimens and associated technology

Provided herein are methods of staining biological material for the purpose of detecting, and in some examples also identifying, microorganisms. Methods of Gram staining bacteria using a slow-acting decolorizing formulation, such as one that includes 1,2-propandiol or ethylene glycol, can be used to extend the time of the decolorizing step, and thus permit automation of the Gram staining method. Also provided are compositions and kits for performing automated Gram staining on microscope slides.

Gram staining method with improved decolorization of the crystal violet-iodine complex from gram negative bacteria

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Tucson, AZ, United States of America

Charles H Weidner