Nanogen, Inc.

Gamida for Life B.v.

Charles H Greef

John R Havens

Daniel E Raymond

Michael James Heller

Howard Reese

Gregory J Kevorkian

Michael K Krihak

Thomas J Onofrey

Regina Rooney

Kristie L Lykstad

Jain Krotz

John J Scott

Methods of addressing a biomolecule to a selectively addressable electrode are described. A permeation layer overlying a plurality of selectively addressable electrodes is provided. The permeation layer includes a reactive group that is adapted to bond to a biomolecule and that requires activation through a chemical transformation before bonding to the biomolecule. At least one selectively addressable electrode is biased such that a pH change occurs in an overlying solution of the at least one selectively addressable electrode. The reactive group in a portion of the permeation layer above the at least one selectively addressable electrode is then chemically transformed to an activated reactive group as a result of the pH change. A biomolecule is then bound to the permeation layer overlying the at least one selectively addressable electrode through the activated reactive group.

Biomolecular attachment sites on microelectronic arrays and methods thereof

The present invention pertains to a method of, and a device created by, depositing an inorganic permeation layer on a micro-electronic device for molecular biological reactions. The permeation layer is preferably sol-gel. The sol-gel permeation layer can be created with pre-defined porosity, pore size distribution, pore morphology, and surface area. The sol-gel permeation layer may also function as the attachment layer of the micro-electric device.

Inorganic permeation layer for micro-electric device

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Ramona, CA, United States of America

Charles H Greef