E.i. Dupont De Nemours and Company

Butamax Advanced Biofuels LLC

Genencor International, Inc.

Other

Butamax (Tm) Advanced Biofuels LLC

Charles Edwin Nakamura

Vasantha Nagarajan

Andrew C Eliot

Lisa Anne Laffend

Lori Ann Maggio-Hall

Gail K Donaldson

Michael G Bramucci

Jean-Francois Tomb

Robert Ray Burch

David L Anton

Robert Di Cosimo

Robert R Dorsch

John R Moran

Mark Scott Payne

Gregory Marshall Whited

Perry G Caimi

Stephen K Picataggio

Donald Eugene Trimbur

Sharon Loretta Haynie

Ramesh V Nair

Wonchul Suh

Anthony Arthur Gatenby

Lixuan Lisa Huang

Maria R Diaz-Torres

Lisa L Huang

Amy Kuang-Hua Hsu

Richard D La Reau

Stephen Kenneth Picataggio

From a bacterial strain isolated from an environmental sample, after enrichment in medium containing 1-butanol as the carbon source, a new enzyme with butanol dehydrogenase activity was identified. The enzyme can convert butyraldehyde to 1-butanol, isobutyraldehyde to isobutanol, as well as 2-butanone to 2-butanol and thus is useful for biosynthesis of butanol in recombinant microbial hosts producing these substrates. The encoding gene, named sadB, was isolated from the strain identified as an isolate of

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Methods for the fermentive production of four carbon alcohols is provided. Specifically, butanol, preferably 1-butanol is produced by the fermentive growth of a recombinant bacterium expressing a 1-butanol biosynthetic pathway.

Fermentive production of four carbon alcohols

Methods for the fermentive production of four carbon alcohols are provided. Specifically, butanol, preferably 2-butanol is produced by the fermentive growth of a recombinant bacteria expressing a 2-butanol biosynthetic pathway. The recombinant microorganisms and methods of the invention can also be adapted to produce 2-butanone, an intermediate in the 2-butanol biosynthetic pathways disclosed herein.

Recombinant host cell comprising a diol dehydratase

Methods for the fermentive production of four carbon alcohols are provided. Specifically, butanol, preferably 2-butanol is produced by the fermentive growth of a recombinant bacteria expressing a 2-butanol biosynthetic pathway. The recombinant microorganisms and methods of the invention can also be adapted to produce 2-butanone, an intermediate in the 2-butanol biosynthetic pathways disclosed herein. Specifically disclosed herein are the use of coenzyme B-independent butanediol dehydratases that catalyzes the substrate to product conversion of 2,3-butanediol to 2-butanone in the process of producing 2-butanol and 2-butanone.

A process is provided for the bioconversion of a carbon substrate to 1,3-propanediol by a single organism utilizing microorganisms containing the genes encoding for an active glycerol or diol dehydratase enzyme by contacting these organisms with a carbon substrate under the appropriate fermentation conditions.

Bioconversion of a fermentable carbon source to 1,3-propanediol by a single microorganism

A process has been developed for the utilization of fructose by PTSmicroorganisms. Providing the microorganisms with increased fructokinase activity, and optionally with increased fructose transport capacity, allows the microorganisms to use fructose as a nutrient supply with sustained growth. The microbial production of substances such as cell mass, glycerol, and 1,3-propanediol may be achieved during growth in a fructose containing medium using this process.

Utilization of fructose in microbial production strains

A new polypropylene terephthalate composition is provided. The polypropylene terephthalate is comprised of 1,3-propanediol and terephthalate. The 1,3-propanediol is produced by the bioconversion of a fermentatble carbon source, preferable glucose. The resulting polypropylene terephthalate is distinguished from petrochemically produced polymer on the basis of dual carbon-isotopic fingerprinting which indicates both the source and the age of the carbon.

A process is provided for the bioconversion of a carbon substrate to 1,3-propanediol by a single organism utilizing microorganisms, such as,and, containing the genes encoding for an active glycerol or diol dehydratase enzyme by contacting these organisms with a carbon substrate under the appropriate fermentation conditions. Specifically,and,provide the source of exogenous genes for such active dehydratase enzyme.

Processes for the bioconversion of a fermentable carbon source to 1,3-propanediol by a single microorganism

Method for identifying the source of carbon in 1,3-propanediol

A process is provided for the bioconversion of a carbon substrate to 1,3-propanediol by a single organism utilizing either microorganisms containing the genes encoding for an active glycerol or diol dehydratase enzyme by contacting these organisms with a carbon substrate under the appropriate fermentation conditions.

Bioconversion of a fermentable carbon source to 1,3-propanediol by a

Recombinant organisms are provided comprising genes encoding glycerol-3-phosphate dehydrogenase, glycerol-3-phosphatase, glycerol dehydratase and 1,3-propanediol oxidoreductase activites useful for the production of 1,3-propanediol from a variety of carbon substrates.

Method for the production of 1,3-propanediol by recombinant

A process is provided for the bioconversion of glycerol to 1,3-propanediol in which genes from a bacteria known to possess a diol dehydratase enzyme for 1,2-propanediol degradation are cloned into a bacterial host and the host is grown in the presence of glycerol; expression of the foreign genes in the host cell facilitates the enzymatic conversion of glycerol to 1,3-propanediol which is isolated from the culture.

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Claymont, DE, United States of America

Charles Edwin Nakamura