University of Rochester

Charles E Sparks

Michael R Violante

Arthur Jay Moss

Daniel H Ryan

Janet D Sparks

James P Corsetti

Described is a method of determining the relative risk of an outcome based on an analysis of multiple risk factors. A graphical method is used to take values corresponding to risk parameters and an event outcome to produce a smoothed surface map representing relative risk over an entire space defined by n risk factors. Applying a query data point to the surface map permits the determination of the estimated outcome probability for the query data point, based on its location on the surface map. The method then reports a relative risk or other probability measure associated with the query data point. Also described is a method of analysis in which subpopulations previously identified as high-risk can be further analyzed with respect to risk posed by additional factors.

Identifying risk of a medical event

The invention relates to a process for preparing standards and controls for immunoassays employing monoclonal antibodies. Monoclonal antibodies are used to isolate a restricted portion of an antigen containing an epitope that determines the specificity of the monoclonal antibody-antigen reaction so as to distinguish it from the antigen as a whole, following fragmentation of the complex antigen by procedures including proteolysis. Isolated epitopes are attached covalently or by physical adsorption to particles to immobilize and stabilize the epitope. The particles can be composed of iodipamide ethyl ester, polyvinyl chloride, polystyrene and other inert substances and can be chemically activated to improve epitope binding and stability. Experimental details demonstrate the binding of lipoprotein epitopes to IDE, polyvinyl chloride and polystyrene and the subsequent reaction of monoclonal antibodies to these particle-stabilized epitopes. This invention is useful particularly for measuring the lipoprotein components associated with cholesterol, and includes a monoclonal based immunoassay kit for measuring the lipoprotein components in biological fluids to assess risk related to coronary artery disease complications.

Growing community of inventors

Pittsford, NY, United States of America

Charles E Sparks