Savannah River Nuclear Solutions, LLC

Battelle Savannah River Alliance, LLC

Charles E Milliken

Scott D Greenway

Charles E Turick

Hector R Colon-Mercado

Ariane L Martin

Hector Colon-Mercado

The present invention is directed to a method of detecting microbial stress. The method comprises the following: providing an electrochemical device having at least one reference electrode and one working electrode wherein the electrochemical device also contains a fermenting microbe, setting an electrochemical potential, providing a source of electrical energy electrically connected to the at least one working electrode, detecting a transfer of electrons from the working electrode to the fermenting microbe, wherein the detection is an indication of microbial stress, and providing a remedial action in response to the indication of microbial stress.

Electrochemical detection of microbial stress

Systems and methods for monitoring microorganisms on a surface are provided. In particular, a flat, patterned sensing electrode can be positioned proximate a surface in non-contact relationship with the surface. The sensing electrode can include a working electrode and a counter electrode. The surface and the sensing electrode can be submerged in an aqueous medium. An alternating current signal can be applied at the working electrode. The signal can propagate through the aqueous medium and can be measured at the counter electrode. The presence of microorganisms on the surface can cause changes in the signal as the signal propagates through the aqueous medium. Such changes in the signal can be used to determine impedance parameters, which can correspond to microbial characteristics associated with the surface. For instance, the microbial characteristics can be associated with a biofilm, corrosion and/or bio-corrosion on the surface.

Non-contact monitoring of biofilms and corrosion on submerged surfaces with electrochemical impedance spectroscopy

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Evans, GA, United States of America

Charles E Milliken