Synthetic Genomics, Inc.

Charles E Merryman

J Craig Venter

Clyde A Hutchison

John Irvin Glass

Carole Lartigue

Sanjay Vashee

Hamilton O Smith

Daniel Glenn Gibson

Ray-Yuan Chuang

Vladimir N Noskov

Gwynedd A Benders

Mikkel A Algire

John I Glass

Nina Alperovich

Daniel G Gibson

The presently disclosed invention relates to methods of installing a genome isolated from one species (the donor) into suitably prepared cells of a second species (the recipient). Introduction of the donor genetic material into the recipient host cell effectively converts the recipient host cell into a new cell that, as a result of the operation of the donated genetic material, is functionally classified as belonging to the genus and species of the donor genetic material.

Methods of genome installation in a recipient host cell

Compositions and methods are disclosed herein for cloning a donor genome in a heterologous host cell. In one embodiment, the donor genome can be further modified within a host cell. Modified or unmodified genomes can be further isolated from the host cell and transferred to a recipient cell. Methods disclosed herein can be used to alter donor genomes from intractable donor cells in more tractable host cells.

Methods for cloning and manipulating genomes

Compositions and methods are disclosed herein for cloning a synthetic or a semi-synthetic donor genome in a heterologous host cell. In one embodiment, the donor genome can be further modified within a host cell. Modified or unmodified genomes can be further isolated from the host cell and transferred to a recipient cell. Methods disclosed herein can be used to alter donor genomes from intractable donor cells in more tractable host cells.

Growing community of inventors

Sykesville, MD, United States of America

Charles E Merryman