Miles Laboratories, Inc.

Charles E Brothers

Chong Y Kim

Jack W Brewer

Terry F Farver

James E Slager

Eunkyu Lee

Extracellular enzymes can be recovered from a whole fermentation beer by adding to the whole beer a mixture of a polymer and an inorganic salt. This total mixture produces an enzyme-rich polymer phase and a cell debris-containing, enzyme-poor salt phase which can be separated to produce an enzyme-rich product from the polymer phase.

Method for the recovery of extracellular enzymes from whole fermentation

This invention relates to a novel process for the recovery of enzymes obtained from enzyme-producing microorganisms, and to the liquid enzyme product recovered by this process. Typically, the enzyme-containing filtrate from a fermentation of an enzyme-secreting microorganism is concentrated and a precipitation agent such as a salt or an organic solvent is added to the concentrate, thereby forming a cake. Then, a polyol solvent is circulated through the cake to solubilize the enzyme or enzyme complex from the cake and provide a liquid enzyme product. Particularly effective is propylene glycol as the polyol solvent. The liquid enzyme product may be shipped as is or subjected to further treatment to remove the solvent and create an essentially solvent-free enzyme product. The process is especially effective for the recovery of alkaline protease or alpa amylase.

Process to solubilize enzymes and an enzyme liquid product produced

The present invention involves a method for the preparation of the title compound which method involves reacting, in a suitable solvent, 1,4,5,8-tetrahydronaphthalene or 9,10-dihydroxy-1,4,5,8-tetrahydronaphthalene with IO.sub.4.sup.- in the presence of a catalytic amount of RuO.sub.2. The title compound is useful as a chelating agent.

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Cassopolis, MI, United States of America

Charles E Brothers