Becton Dickinson and Company Limited

Other

Charles C Yu

Timothy M Wiles

Mei Yang-Woytowitz

Craig C Whiteford

Michael A Brasch

Brent Ronald Pohl

Patrick Shawn Beaty

Ming-hsiung Yeh

Liping Feng

Ben Turng

Gary F Hershner

Eric Ursprung

Xiaofei Chang

Patrick R Murray

Dwight Livingston

Ammon David Lentz

Kevin Bailey

Gerard Lotz

Ming-Hsiung Yeh

An apparatus and associated methods of use for a controlled combination of reagents is disclosed. The apparatus includes a vessel, a vessel insert, and a cap element. The vesselhas a body portionfor receiving a biological sample. The vessel insertreceives at least one reagent therein. Preferably, the vessel insertis received in a portionof the vessel. The cap elementis attached to the vesselto secure the vessel insertin the vessel. During use, the vessel insertis adapted to release its contents when the biological sample is introduced into the body portionof the vesselupon application of an intermixing force to the vessel insert. A variety of intermixing forces may be applied, depending upon the embodiment of the present invention and its associated methods of use.

Blood culture bottles with mechanisms for controlled release of substances into culture media

Presented herein are methods, compositions, and kits for the detection of specific beta-lactamases, including class A serine carbapenemases, metallo-beta-lactamases, AmpC beta-lactamases, and extended-spectrum beta-lactamases (ESBLs). The methods presented herein include methods that permit the detection of the presence of specific beta-lactamases in bacterial samples within as few as 2 to 10 minutes.

Kits for the detection of beta-lactamases

Presented herein are methods for the detection of specific beta-lactamases, including class A serine carbapenemases, metallo-beta-lactamases, AmpC beta-lactamases, and extended-spectrum beta-lactamases (ESBLs). The methods presented herein include methods that permit the detection of the presence of specific beta-lactamases in bacterial samples within as few as 2 to 10 minutes.

Methods for the detection of beta-lactamases in a sample

Presented herein are solid supports on or in which a composition comprising a lysis reagent and an intracellular microorganism target detection reagent (e.g., an intracellular bacterial target detection reagent) is present in a dried form. Presented herein are also methods for detecting the presence of an intracellular microorganism target (e.g., an intracellular bacterial target) utilizing such solid supports. Further, presented herein are tablets as well as dry powders comprising a lysis reagent and an intracellular microorganism target detection reagent (e.g., an intracellular bacterial target detection reagent), and methods of using such tablets and dry powders to detect the presence of an intracellular microorganism target (e.g., an intracellular bacterial target).

Compositions for the detection of intracellular bacterial targets and other intracellular microorganism targets

Presented herein are methods and compositions for the detection of specific beta-lactamases, including class A serine carbapenemases, metallo-beta-lactamases, AmpC beta-lactamases, and extended-spectrum beta-lactamases (ESBLs). The methods presented herein include methods that permit the detection of the presence of specific beta-lactamases in bacterial samples within as few as 2 to 10 minutes.

Methods for the detection of beta-lactamases

Presented herein are solid supports on or in which a composition comprising a lysis reagent and an intracellular microorganism target detection reagent (e.g., an intracellular bacterial target detection reagent) is present in added form. Presented herein are also methods for detecting the presence of an intracellular microorganism target (e.g., an intracellular bacterial target) utilizing such solid supports. Further, presented herein are tablets as well as dry powders comprising a lysis reagent and an intracellular microorganism target detection reagent (e.g., an intracellular bacterial target detection reagent), and methods of using such tablets and dry powders to detect the presence of an intracellular microorganism target (e.g., an intracellular bacterial target).

Compositions for the detection of intracellular bacterial targets and other intracellular micororganism targets

Presented herein are kits for the detection of specific beta-lactamases, including class A serine carbapenemases, metallo-beta-lactamases, AmpC beta-lactamases, and extended-spectrum beta-lactamases (ESBLs). The kits presented herein include kits that permit the detection of the presence of specific beta-lactamases in bacterial samples within as few as 2 to 10 minutes.

Compositions and methods for the rapid and sensitive detection of a carbapenemase in a sample are provided. The compositions include novel primer and probe compositions for use in detecting the presence of this enzyme in a sample, particularly using PCR methods. These primers and probe sets can be used in amplification methods (such as PCR, particularly quantitative PCR) and packaged into kits for use in amplification methods for the purpose of detecting carbapenemase in a test sample, particularly a patient sample, particularly a direct sample. Thus, in one embodiment, the present invention provides for novel oligonucleotide primers set forth in SEQ ID NOs:1, 2, 4, 5, 7, 8, 14, 15, 17, 18, and 20, and the novel oligonucleotide probe sequences set forth in SEQ ID NOs:3, 6, 9, 16, and 19. These sequences can be used in a method of detecting carbapenemase in a sample.

Methods for treating infection involving identification of a carbapanemase gene

Compositions and methods for the identification of a carbapenemase gene

Presented herein are methods and compositions for the detection of specific beta-lactamases, including class A serine carbapenemases, metallo-beta-lactamases. AmpC beta-lactamases, and extended-spectrum beta-lactamases (ESBLs). The methods presented herein include methods that permit the detection of the presence of specific beta-lactamases in bacterial samples within as few as 2 to 10 minutes.

Compositions and methods for the rapid and sensitive detection of a carbapenemase in a sample is provided. The compositions include novel primer and probe compositions for use in detecting the presence of this enzyme in a sample, particularly using PCR methods. These primers and probe sets can be used in amplification methods (such as PCR, particularly quantitative PCR) and packaged into kits for use in amplification methods for the purpose of detecting carbapenemase in a test sample, particularly a patient sample, particularly a direct sample. Thus, in one embodiment, the present invention provides for novel oligonucleotide primers set forth in SEQ ID NOs:1, 2, 4, 5, 7, 8, 14, 15, 17, 18, and 20, and the novel oligonucleotide probe sequences set forth in SEQ ID NOs:3, 6, 9, 16, and 19. These sequences can be used in a method of detecting carbapenemase in a sample.

An improved endotracheal tube is disclosed which provides for a more reliable and safer airway to a patient's lungs for the purpose of administering anesthesia, supplying oxygen and assisting a patient in breathing, and the like. The new and improved features of the tube make it easier for a health care professional to secure the tube to a patient's face, monitor its depth of insertion into the throat and assure its easy connection an disconnection from a medical or surgical apparatus. These improved features include flexible flanges molded to the tube, each of said flanges including a plurality of radially opposite slots to which adhesive tape is attached for securing said tube to a patient's face. The tube is also provided with colored rings, located between the upper and lower edges of each set of slots for monitoring the depth of insertion of the tube into the throat of a patient. A connection end of the tube is fitted with internal threads for threadedly engaging a universal connector for attachment to a medical or surgical apparatus.

Growing community of inventors

Lutherville, MD, United States of America

Charles C Yu