Eastman-kodak Company

Johnson & Johnson Clinical Diagnostics, Inc.

Charles C Hinckley

John Bruce Findlay

William Harold Donish

Jeffrey A Wellman

Paul Nicholas Schnipelsky

Leonard J Seaberg

Dennis R Zander

Scott H Schwallie

Thomas J Cummins

John B Chemelli

Craig A Caprio

Sheryl S Sullivan

Richard W Bacchetta

Thomas C Littlefield

Lewis T Del Plato, Jr

Michael R Van Der Gaag

A cuvette and a method of use prevent nucleic acid amplified by PCR technology from being released to the atmosphere, while still proceeding to a detection step to determine whether or not the nucleic acid is present. Detection reagents are either pre-incorporated into compartments in the cuvette or added after amplification. In the latter case, a check valve prevents amplified nucleic acid from being released. Transfer of liquids between compartments is achieved via the use of flexible compartment walls and an external pressure source, or via pistons that are part of the cuvette and operate on the compartments as a piston within a piston chamber.

Containment cuvette for PCR and method of use

A heating assembly useful in apparatus for processing reaction cuvettes for replicating specified DNA sequences, such as those using PCR, having a heating element with a heat delivering surface for compressively contacting a pliable fluid-carrying compartment of a supported cuvette. The heat delivering surface has a defined passage sized to allow the detection compartment to be situated therein so that the compartment can be efficiently heated. Fluid flow through the compartment, however, is not interfered with during the heating process due to the presence of the defined passage. In addition, the heat delivering surface can be made from optically transparent materials so that visual detection within the processor can take place.

Apparatus for heating a fluid-carrying compartment of reaction cuvette

A cuvette and a method of use which prevent nucleic acid amplified by PCR technology from being released to the atmosphere, while still proceeding to a detection step to determine whether or not the nucleic acid is present. Detection reagents are either pre-incorporated into compartments in the cuvette or added after amplification. In the latter case, a check valve prevents amplified nucleic acid from being released. Transfer of liquids between compartments is achieved via the use of flexible compartment walls and an external pressure source, or via pistons that are part of the cuvette and operate on the compartments as a piston within a piston chamber.

There is described a container used to extract antigens for an immunoassay, having a wall means defining open and closed opposite ends. The container is provided with dividing members located in the closed end of the container which divides the volume of the closed end into at least two separate regions. A portion of the container's wall means adjacent to the closed end is shaped and sized to cooperate with a depositing device to ensure that separate reagents are separately deposited on opposite sides of the dividing members.

Extracting device for extracting antigens

A filter providing at least two different filtering pore sizes for coarse and fine filtering, and a kit containing such filter along with an immunoassay test device containing a membrane. The membrane is used to separate bound immunoassay labels from free labels. The coarse and fine filtering are provided preferably by two different, serially arranged filters, the filter with the fine pore size being selected with a pore size similar to that of the membrane of the assay device.

Multiple level filter device and kit containing same

There is disclosed a liquid-collecting compartment that is vented to allow air to escape. The compartment is improved in that a closure member is provided constructed so as to be slidable onto and off of the vent aperture with a force that is between about 0.15 newtons and about 20 newtons. To achieve this, an elastomeric member is provided in the closure member or the wall at the aperture, with selected properties of durometer, cold flow and static coefficient of friction against polystyrene.

Sliding valve for vent of liquid collecting compartment

There is disclosed a container for storing a reagent and then for reacting the reagent to produce a signal representative of the presence of an analyte, or of the amount of that analyte. The container is disposable and comprises at least one compartment having an upper portion and a lower portion. The upper portion comprises a stored reagent and means for retaining for observation on an indicator surface a reaction product of such reagent following a reaction of the reagent with a liquid sample. The lower portion includes means for absorbing liquid extracted from the upper portion through the retaining means. The absorbing means is configured with a shape that contacts the confining means only at locations spaced inwardly away from at least two of the side walls of the upper portion, whereby the reaction product of the liquid sample and the reagent is induced to flow into the confining means away from the two side walls to produce a uniform signal.

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Pittsford, NY, United States of America

Charles C Hinckley