Centre National De La Recherche Scientifique

Ecole Polytechnique

Commissariat &#xc0; L'&#xc9;nergie Atomique Et Aux &#xc9;nergies Alternatives

Centre National De La Recherche Scientifique-cnrs

Charles Baroud

Rémi Dangla

Paul Abbyad

Silvan Turkcan

Jean-Pierre Delville

Raphaël Tomasi

Étienne Fradet

Laurent Mugherli

Francois Gallaire

Gabriel Amselem

Régis Wunenburger

Patrick Huerre

Sébastien Sart

Raphael Tomasi

François Gallaire

The subject matter of the present invention is a microfluidic process for treating and analysing a solution containing a biological material, comprising a step of introducing the solution into microchannels of a microfluidic circuit (), a step of forming drops of this solution, under the effect of modifications of the surface tension of the solution, a step of moving the drops to one or more drop storage zones(s) (), under the effect of modifications of the surface tension of the drops, a step of treating the drops and a step of analysing the drops.

Microfluidic process for treating and analysing a solution containing a biological material and corresponding microfluidic circuit

Method for handling at least one first microdrop and at least one second microdrop in a microfluidic system including a capillary trap that has a first trapping zone and a second trapping zone, the method including steps consisting of: (i) trapping the first microdrop in the first trapping zone, and (ii) trapping the second microdrop in the second trapping zone, the first and the second trapping zone being arranged such that the first and the second microdrops are in contact with each other, the first and the second trapping zones being adapted such that the trapping forces returned to one of the microdrops are different.

Microfluidic method for handling microdrops

A method for handling, in a microfluidic system, microdrops which include samples, including the steps of forming, in an oil, microdrops of an aqueous solution containing a sample, the oil and/or the aqueous solution containing a sample including a gelling agent; trapping the microdrops by means of surface-tension traps pre-arranged in a trapping area; and at least partially gelling the oil in the trapping area and/or at least partially gelling the trapped microdrops.

Method for handling microdrops which include samples

The subject of the present invention is a microfluidic circuit in which are defined microchannels able to contain fluids and including at least one device for forming drops of a solution, guiding the drops to a storage zone in which one of the drops can be brought into contact with a drop of another solution, the walls of the microchannel portion forming the first drop-formation device diverging so as to cause drops of the first solution to detach under the effect of the surface tension of the first solution; the first guide include wall portions of the microchannels that diverge so as to cause the drops to move along under the effect of the surface tension of the first solution.

Microfluidic circuit allowing drops of several fluids to be brought into contact, and corresponding microfluidic method

Provided herein are microfluidic circuits that include at least one device for forming a quantity of drops of a solution in a carrier fluid and at least one storage zone for storing drops produced by the microfluidic device. Such microfluidic circuits are useful, for example, for the analysis of a solution containing a biological sample.

The invention relates to a microfluidic circuit including at least one microchannel for the flow of a first fluid conveying drops or bubbles of at least one second fluid, characterized in that the height of the microchannel is sized so as to crush the drops or bubbles during the movement thereof, and in that the microchannel comprises at least one trough, extending at least partially in the direction of flow of the first fluid or an area for trapping drops or bubbles, said area or the trough having a height that is greater than the height of the microchannel, such that at least some of the drops or bubbles of the second fluid in the microchannel are drawn and guided into the trough or into the trapping area.

Microfluidic circuit

The invention relates to a device () for forming droplets in a microfluidic circuit, the device comprising a chamber () containing a first fluid and defined by two opposite walls () that diverge relative to each other in at least one given direction, and a microchannel () containing a second fluid and leading into a zone of said chamber () that is upstream relative to the given direction, the outlet of the microchannel () into the chamber () constituting an enlargement in the flow section for the second fluid, and the enlargement giving rise to droplets () of the second fluid forming within the first fluid.

Device for forming drops in a microfluidic circuit

The invention relates to a method for treating drops in a microfluid circuit, comprising at least one microchannel () through which the drops flow, characterized in that a laser () is brought to bear on the interface of said drops in the transport liquid (F), or on the interface of drops in contact, in order to carry out a sorting of the drops, to form nanodrops from a larger drop or to fuse drops () in contact and initiate reactions between the fluids contained in said drops.

Method for treating drops in a microfluid circuit

A microfluidic circuit comprising microchannels () containing different fluids (F, F), with a laser beam being focused at () on an interface () between the fluids so as to form a pump, a valve, or a mixer, for example.

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Paris, France

Charles Baroud