Archer Daniels Midland Company

Battelle Memorial Institute

Charles Abbas

Kyle E Beery

Andriy Sibirny

Andriy Voronovsky

Thomas P Binder

Anne M Rammelsberg

Olena Ishchuk

Perry H Doane

Michael J Cecava

Leif Solheim

John G Frye

Todd A Werpy

Alan H Zacher

Dan L Fanselow

Gary G Neuenschwander

Andrew J Schmidt

Rick J Orth

David P Holzgraefe

James A Franz

Kostyantyn Dmytruk

Mikhail S Alnajjar

Eric V Alderson

Catherine J Kim

Wu-Li Bao

Barbara Kshanovska

Robert T Fitzsimons, Jr

John F Reed

Masayuki Nakamura

Mark G Matlock

Leif P Solheim

Matt Dyer

Andrii Y Voronovsky

Andrii A Sibirny

Kateryna A Sibirna

Kostiantyn V Dmytruk

Liubov R Fayura

Olena B Ryabova

Andriy Y Voronovsky

Oleh V Stasyk

Dan L Brown

Mark D Isder

Travis Nelson

Brian Bone

Rhea J Sammons

Jerry Barnes

Jerod Fults

Olena Dmytruk

John Reed

Andriy A Sibirny

Olena P Ishchuk

Dan Fanselow

Robert Fitzsimons

Mark Isder

Masa Nakamura

Lidiia R Murashchenko

Kostyantyn V Dmytruk

The present invention is directed to a system and method for producing an organic compound using fermentation wherein multiple components of the system are recycled within the system. The system and method allow for extraction of a high concentration of the organic compound from the fermentation broth in a continuous system that allows recycling of the biomass, aqueous fermentation broth and extraction solvents. The system and method are particularly well adapted for producing and extracting ethanol.

System and method for extracting ethanol from a fermentation broth

Methods of identifying genes conferring ethanol tolerance in yeasts, genes that confer ethanol tolerance, and mutant strains used to identify such genes are described. A gene herein designated HpETT1 was isolated from the yeast. Expression of HpETT1 in an ethanol sensitive mutantstrain designated 7E complimented ethanol sensitivity of the mutant. When multiple copies of the HpETT1 were integrated into the genome and overexpressed, the transformed strain demonstrated approximately 10-fold greater resistance to ethanol and resistance to the protein misfolding agent AZC. Expression of HpETT1 also increased ethanol tolerance in. HpEtt1 has 39% sequence identity to a previously identified protein fromdenoted MPE1, however, the MPE1 gene does not confer ethanol resistance to the 7E mutant. Another gene from the yeastwas identified that encodes an orthologue protein having 37% identity to HpETT1 herein designated PsETT1 and also confers ethanol resistance to the 7E mutant.

Genes conferring tolerance to ethanol and high temperature for yeasts

Described herein are new approaches for the selection ofstrains with increased ethanol production from hydrolyzed starch derived sugars. An industrial production strain ofAS400 was subjected to positive selection of mutants resistant to toxic concentrations of oxythiamine, trehalose, 3-bromopyruvate, glyoxylic acid, and glucosamine. The selected mutants are characterized by 5-8% increase in ethanol yield (g gof consumed glucose) as compared to the parental industrial ethanol-producing strain. A multiple-step selection approach that consisted of the sequential selection using glyoxylic acid, glucosamine and bromopyruvate as selective agents resulted in a 12% increase in ethanol yield during fermentation on industrial media. These results indicate that the selection methods provided herein are useful for producing a variety of strains that are promising candidates for industrial ethanol production.

Methods for the positive selection of ethanol overproducing mutants from

A method to increase ethanol production from a corn dry-mill process is described that comprises adding an enzyme preparation derived fromhaving cellulolytic activity to a saccharification process that includes conventional alpha amylase and glucoamylase. The addition of the cellulolytic enzyme decreases viscosity of the saccharified mash and can increase ethanol yield from a dry grind fermentation by as much as 10% or more. Specific characteristics are provided to show surprising and advantageous results of one particular preparation of cellulolytic enzymes from

Cellulolytic enzyme enhancement of dry grind corn processing and ethanol production

Genes SWA2 and GAM1 from the yeast,, encoding &#x3b1;-amylase and glucoamylase, respectively, were cloned and expressed in. The expression was achieved by integration of the SWA2 and GAM1 genes into the chromosome ofunder operably linked to a strong constitutive promoter of the-glyceraldehyde-3-phosphate dehydrogenase gene (HpGAP. Resulting transformants acquired the ability to grow on a minimal medium containing soluble starch as a sole carbon source and can produce Ethanol at high-temperature fermentation from starch up to 10 g/L. A XYN2 gene encoding endoxylanase was obtained from the fungus, and a xlnD gene coding for &#x3b2;-xylosidase was obtained from the fungus. Co-expression of these genes was also achieved by integration into thechromosome under control of the HpGAP promoter. The resulting transformants were capable of growth on a minimal medium supplemented with birchwood xylan as a sole carbon source. Successful expression of xylanolytic enzymes resulted in a recipient strain capable of fermentation of birchwood xylan to ethanol at 48&#xb0; C. Further with co expression of the forgoing genes in astrain that overexpresses a pyruvate decarboxylase gene further improved ethanol production.

[object Object]

Recombinant genetic constructs and strains ofhaving significantly increased ethanol productivity with a simultaneous decreased production of xylitol during high-temperature xylose fermentation are disclosed. The constructs include aXYL1 gene encoding xylose reductase mutated to decrease affinity of the enzyme toward NADPH. The modified version of XYL1 gene under control of a strong constitutive HpGAP promoter was overexpressed in a &#x394;xyl1 background. A recombinantstrain overexpressing the mutated enzyme together with native xylitol dehydrogenase and xylulokinase in the &#x394;xyl1 background was also constructed. Xylose consumption, ethanol and xylitol production by the constructed strain were evaluated during high-temperature xylose fermentation (48&#xb0; C.). A significant increase in ethanol productivity (up to 7.4 times) was shown in the recombinant strain as compared with the wild type strain. Moreover, the xylitol production by the recombinant strain was reduced considerably: 0.9 mg&#x3c7; (L&#x3c7;h)versus 4.2 mg&#x3c7; (Lxh)by the wild type strain.

Novel fiber processing methods and the products obtained therefrom are disclosed. Methods may include thermochemical and/or enzymatic hydrolysis of fiber feedstocks including distillers' dried grains, distillers' dried grains with solubles, soyhull, miscanthus and switchgrass. Enzymatic hydrolysis includes hydrolysis with cellulase, hemicellulase, and protease.

Increased fiber hydrolysis by protease addition

Methods and compositions for the production of ethanol from lignocellulosic starting materials are provided herein. Embodiments provide yeast cells of the genuswith one or more modifications, including, for example, an inactive acid trehalase gene, overexpression of xylulokinase, and/or overexpression of heat-shock protein 104.

The present invention relates to the use of 'green' or relatively benign solvents such as ethanol, ethanol/water, isopropyl alcohol, isopropyl alcohol/water, ethyl lactate, acetone, butanol, isoamyl alcohol, or ethyl acetate to extract phytosterols from wet corn fiber. The resulting oil product contains free phytosterols and free fatty acids.

Extraction of phytosterols from corn fiber using green solvents

Methods and compositions for the production of ethanol from lignocellulosic starting materials are provided herein. Embodiments of the invention provide methods of manipulating the carbon flux of a host cell transformed with plasmids of the invention. Plasmids of the invention may include nucleotides that encode pyruvate decarboxylase. In one embodiment, a strain of the thermotolerant yeastthat has been transformed with plasmids and polynucleotides of the invention is provided.

Increased ethanol production from xylose

A method for producing hydrogen from a plant source is disclosed. The method includes contacting a crude carbohydrate material obtained from the plant source with water and a catalyst at a temperature and pressure sufficient to decompose at least a portion of the crude carbohydrate material to form a vapor mixture of gases including hydrogen, and separating hydrogen from other gases present in the vapor mixture.

Process for hydrogen gas production from carbohydrate feedstocks

A method for the production of ethanol and a modified animal feed is provided. The method replaces the starch in known corn-based animal feed with biomass fiber treated to make it more digestible by animals. The process includes wherein the pericarp and germ are removed from the corn kernel and processed for by-products. The starch and protein are also removed and separated. The starch is then fermented and distilled to ethanol and stillage. The bioavailable modified animal feed comprises the pericarp and germ removed from corn kernels and optionally by-products of the pericarp and germ processing, and lignocellulosic materials. The modified animal feed may optionally include energy materials such as animal and vegetable fats, vegetable soapstocks, or glycerin, and combinations thereof.

Process for the production of animal feed and ethanol and novel animal feed

Growing community of inventors

Champaign, IL, United States of America

Charles Abbas