E.i. Dupont De Nemours and Company

Charles A Omer

Daniel P O'Keefe

Fateme Sima Sariaslani

Caroline Dean

Kenneth Joseph Leto

James A Romesser

Patricia A Harder

James M Tepperman

Paul Veikko Viitanen

Michael K Trower

Steven E Buchholz

The present invention provides a method of making a recombinant organism capable of oxidizing organic chemicals by constitutive production of proteins capable of performing oxidation. A recombinant organism and a method of oxidizing organic chemicals are also provided. The present invention is useful in bioremediation to remove waste chemicals from the environment.

Constitutive expression of P450soy and ferredoxin-soy in Streptomyces

DNA sequences encoding herbicide metabolizing cytochrome P450 enzymes and iron-sulfur proteins that donate electrons to these enzymes, were introduced into plants and microorganisms rendering them able to produce the encoded gene products and to metabolize herbicides.

Expression of herbicide metabolizing cytochromes P450

Expression of herbicide metabolizing cytochromes

A process for inducting cytochrome P-450 enzyme production in bacteria of the genus Streptomyces using inducers such as soybean flour, genistein or genistin is described. Uses for the cytochrome P-450 enzymes produced are also discussed as is a process for using genetically engineered Streptomyces to determine the mutagenicity of chemicals.

Process for inducing cytochrome P-450 enzymes in streptomyces bacteria

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Downingtown, PA, United States of America

Charles A Omer