Dow Agrosciences LLC

United States of America, as Represented by the Secretary of Agriculture

Charles A Mihaliak

Janis K McMillen

Matthew J Fanton

Donald J Merlo

Steven Robert Webb

Steven Lee Evans

Kelley Ann Smith

Geoffrey J Letchworth

The subject invention provides a plant cell culture for producing proteinaceous agents comprising a plant cell line stably transformed to express a transgene encoding a proteinaceous agent and a growth medium which supports the growth of said plant cell culture but which does not support the growth of Mycoplasmataceae and contains no materials of animal origin. The plant cell line is capable of being continuously passaged such that consistent transgene expression is maintained during passaging. The plant cell line is also capable of being cryopreserved such that consistent transgene expression is recovered upon recovery from cryopreservation.

Preparation of vaccine master cell lines using recombinant plant suspension cultures

The subject application provides various compositions of matter directed to West Nile virus (WNV) polypeptides and fragments thereof and polynucleotides, vectors and transformed host cells that encode, direct the expression of, or produce WNV polypeptides as set forth herein. Methods of using the polypeptides and polynucleotides for the production of immune responses in individuals or detecting the presence of WNV specific or neutralizing antibodies are also provided herein.

Plant-made west nile virus (WNV) vaccines, vectors and plant codon optimized sequences

Growing community of inventors

Apex, NC, United States of America

Charles A Mihaliak