Dionex Corporation

Christopher A. Pohl

Charanjit Saini

Christopher A Pohl

Manikandan Jayaraman

Jacek J Jagodzinski

An ion exchange chromatographic packing material is described that includes support resin particles and a copolymer grafted to the support resin particles. The copolymer includes polymerized functional monomers such as a first ion exchange group monomer and a second ion exchange group monomer. At a first pH, the first ion exchange group monomer is configured to have a first charge at a first pH, and the second ion exchange group monomer is configured to have a net neutral charge. At a second pH, the first ion exchange group monomer is configured to have the first charge at a second pH, and the second ion exchange group monomer is configured to have a second charge at the second pH where the first charge and second charge both have a same polarity.

Ion exchange chromatography column, method, and system thereof

An anion exchange stationary phase includes a negatively charged substrate particle, a base condensation polymer layer, a crosslinked ethanolamine condensation polymer, and a glycidol condensation layer. The crosslinked ethanolamine condensation polymer layer can be covalently attached to the base condensation polymer layer. The crosslinked ethanolamine condensation polymer layer can be formed by a condensation reaction product of a polyepoxide compound and ethanolamine. The glycidol condensation layer can be formed by the treatment of glycidol. The anion exchange stationary phase are suitable for separating a variety of haloacetic acids and common inorganic anions in a single chromatographic run in less than 20 to 30 minutes.

Anion exchange stationary phases based on crosslinked hydroxyalkylamine layer and glycidol

An anion exchange stationary phase includes a negatively charged substrate particle, a base condensation polymer layer, a crosslinked ethanolamine condensation polymer, and a glycidol condensation layer. The crosslinked ethanolamine condensation polymer layer can be covalently attached to the base condensation polymer layer. The crosslinked ethanolamine condensation polymer layer can be formed by a condensation reaction product of a polyepoxide compound and ethanolamine. The glycidol condensation layer can be formed by the treatment of glycidol. The anion exchange stationary phase are suitable for separating a variety of haloacetic acids and common inorganic anions in a single chromatographic run in less than 20 to 35 minutes.

A liquid chromatography agglomerated bed comprising component A comprising (a) substrate particles and polymer chains (e.g. a condensation polymer) bound to the substrate particles and projecting therefrom, and (b) component B comprising substrate particles having external surfaces of opposite charge to that of the charged polymer chains, components A and B being bound at least in part by electrostatic forces between the component A charged polymer chains and the component B external surfaces to form in composite an agglomerated bed of ion exchange particles packed in a chromatography column.

Agglomerated ion exchange particle bed and method

A method for making an ion exchange coating (e.g., a chromatographic medium) on a substrate comprising (a) reacting at least a first amine compound comprising amino groups, with at least a first polyfunctional compound, in the presence of a substrate to form a first condensation polymer reaction product, with a first unreacted excess of either at least said first amino group or polyfunctional compound functional moieties, irreversibly attached to the substrate, and (b) reacting at least a second amine compound or at least a second polyfunctional compound with unreacted excess in the first condensation polymer reaction product to form a second condensation polymer reaction product, and repeating the steps to produce the desired coating. A coated ion exchange substrate so made.

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Fremont, CA, United States of America

Charanjit Saini