Hangzhou Ruifeng Biotechnology Limited Inc.

Chaoyang Lin

Zhicheng Shen

Xiaoli Xu

Chengyi Liu

Xinlan Li

Disclosed are a herbicide resistance gene and use thereof, wherein 1) the amino acid sequence of the encoded protein is at least more than 80% identical to SEQ ID NO:1; and 2) the encoded protein is capable of resulting in resistance to at least one of the following types of herbicides: acetolactate synthase (ALS)-inhibiting herbicides, protoporphyrinogen oxidase (PPO)-inhibiting herbicides, p-hydroxyphenylpyruvate dioxygenase (HPPD)-inhibiting herbicides, photosystem II-inhibiting herbicides, and synthetic auxin herbicides. The gene disclosed herein can be introduced into a plant to obtain a transgenic plant against herbicides. A method is disclosed for obtaining an herbicide-resistant transgenic plant by using an herbicide-resistant gene. Such herbicide-resistant transgenic plant can prevent damage of herbicides to the plant, providing a convenient and economical means for selectively killing weeds.

Herbicide resistance gene and use thereof

Disclosed are a method for obtaining highly glyphosate-resistant G1174 gene mutants through artificial mutation and use thereof. Two or more amino acid mutations are introduced in the amino acid sequence from position 95 to position 114 of protein encoded by G1174 gene, and the highly glyphosate-resistant mutants are selected. Also disclosed is a highly glyphosate-resistant mutated G1174 gene, wherein the amino acid sequence from position 95 to position 114 is selected from any one of SEQ ID NO: 4 to SEQ ID NO: 24. The use of the highly glyphosate-resistant mutated gene is for conferring a plant with glyphosate resistance through expression of the transgene in the plant.

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Hangzhou, China

Chaoyang Lin