Hubei University

Hubel University

Chao Zhai

Lixin Ma

Fei Wang

Jun Yang

Rey-Ting Guo

Jian-Wen Huang

Lilan Zhang

Chun-Chi Chen

Longhai Dai

Xuejing Yu

Chin-Yu Chen

Shan Wu

Zhichun Zhan

A protein complex based on DNA enzymes of an E family ofand an application thereof in artificial protein scaffolds are provided. The protein complex includes one or more of interaction pairs formed by a CL2 protein and an Im2 protein, a CL7 protein and an Im7 protein, a CL8 protein and an Im8 protein, or a CL9 protein and an Im9 protein. By protein engineering of a carboxyl terminus DNase domain of the DNA enzymes CE2, CE7, CE8 and CE9, mutants that lose DNA enzyme activity but still retain the ultra-high affinity with the corresponding Im protein are obtained, and protein interaction pairs CL2/Im2, CL7/Im7, CL8/Im8 and CL9/Im9 are constructed. These protein interaction pairs have properties of heat resistance, high affinity, high specificity, small molecular weight, fast assembly speed, etc. Based on this, an artificial protein scaffold is constructed for the construction of artificial multienzyme complexes.

Protein complex based on DNA enzymes of E family ofand application thereof in artificial protein scaffolds

A cellulase having improved enzymatic activity is disclosed. The cellulase has a modified amino acid sequence of SEQ ID NO: 2 or a modified amino acid sequence with at least 80% sequence identity of SEQ ID NO: 2, wherein the modification is a substitution of methionine at position 120 or a corresponding position with asparagine.

Cellulase having improved enzymatic activity

Growing community of inventors

Wuhan, China

Chao Zhai