General Atomics, Inc.

Diazyme Laboratories, Inc.

Chao Dou

Chong-Sheng Yuan

Abhijit Datta

Fakhri B Saida

Provided herein are compositions and methods for analyzing neutralizing antibodies to SARS-CoV-2, for example, in a sample from a subject suspected of having, having, or having had (e.g., having recovered from) a SARS-CoV-2 infection, or a subject having received a prophylactic and/or therapeutic intervention for a coronavirus infection.

Compositions and methods for assaying neutralizing antibodies

This invention relates generally to the field of homocysteine detection. In particular, the invention provides a method for determining homocysteine presence or concentration in samples, which method comprises: contacting a sample containing or suspected of containing Hcy with a Hcy co-substrate and a Hcy converting enzyme in a Hcy conversion reaction to form a Hcy conversion product and a Hcy co-substrate conversion product; and assessing the Hcy co-substrate conversion product to determine the presence, absence and/or amount of the Hcy in the sample. The Hcy co-substrate conversion product may be assessed directly, or it may be assessed by further conversion of the Hcy co-substrate conversion product into another material by the action of one or more additional enzymes. A kit for assaying homocysteine based on the same principle is also provided.

Methods and compositions for assaying homocysteine

The present invention provides a method for assaying myeloperoxidase activity. In the assay, a sample containing myeloperoxidase or suspected of containing myeloperoxidase is contacted with substrate including serine, hydrogen peroxide, and a halide. If myeloperoxidase is present in the sample, serine is converted into glycolaldehyde, which is further converted into glycolate by a glycoaldehyde converting enzyme. The method then utilizes a cycling reaction system between glycolate and glyoxylate to generate a detectable signal that corresponds to the myeloperoxidase activity. Kits for assaying myeloperoxidases based on the same principle are also provided.

Methods and compositions of enzymatic cycling based assays for myeloperoxidase

This invention relates generally to the field of homocysteine detection. In particular, the invention provides a method for determining homocysteine presence or concentration in samples, which method comprises: contacting a sample containing or suspected of containing Hcy with a Hcy co-substrate and a Hcy converting enzyme in a Hcy conversion reaction to form a Hcy conversion product and a Hcy co-substrate conversion product; and assessing the Hcy co-substrate conversion product to determine the presence, absence and/or amount of the Hcy in the sample. A kit for assaying homocysteine based on the same principle is also provided.

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San Diego, CA, United States of America

Chao Dou