Shaan Xi Lifegen Co., Ltd.

Xi'an Goldmag Nanobiotech Co. Ltd.

Chao Chen

Yali Cui

Jinghui Zeng

Yiwen Gao

Caiquan Zhang

Zheng Li

Longlin Yu

Yitong Tang

Juanli Zhu

Mingli Peng

High-throughput detection for the interesting base or the mutation site in the nucleic acid sample can be achieved by means of the linear test probe pairs P1 and P2. The test probe pairs P1 and P2 respectively comprise either of the flanking complementary sequences which are adjacent to the interesting base or the mutation site in the nucleic acid sample. The invention can be applied to the re-sequencing the target nucleic acid sequence, the detection and analysis for the mutation, insertion, or deletion sites of a known nucleic acid sequence, and the genotyping of the pathogenic microorganism.

Test probes, common oligonucleotide chips, nucleic acid detection method, and their uses

Disclosed is a process of preparing magnetite nanoparticles, comprising the following steps: 1) preparing a ferric salt mixed system, wherein a soluble ferric salt is dissolved in glycol at ambient temperature, and then urea and polyethylene glycol are added and mixed homogeneously to obtain the trivalent iron salt mixed system, the mass ratio of glycol to the trivalent iron salt being 15:1 to 60:1, glycol to urea being 20:1 to 100:1, and glycol to polyethylene glycol being 20:1 to 100:1; 2) reacting, wherein the trivalent iron salt mixed system is transferred into a reaction autoclave, sealed and placed into a heating device to react at a temperature of 200 to 300&#xb0; C. for 8 to 72 hours; and 3) washing, wherein after the reaction system is naturally cooled down to ambient temperature, the product is taken out, and washed with anhydrous ethanol and water in turn to obtain the magnetite nanoparticles. The soluble iron salt includes ferric chloride, ferric sulfate, ferric acetate and ferric nitrate. The obtained nanospheres exhibit a uniform distribution of the particle diameter with a good dispersity in water. The nanospheres have superparamagnetism, and their particle diameter can be controlled by varying the reaction time as desired.

Preparation method of ferroferric oxide magnetic nanospheres

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Shaanxi, China

Chao Chen