Bayer Cropscience Nv

Bayer Bioscience N.v.

Chantal Vanderstraeten

Rene Ruiter

Kathleen D'halluin

Kathleen D'Halluin

Kathleen D′Halluin

Methods and means are provided to improve targeted DNA insertion in plants using rare-cleaving 'double stranded break' inducing enzymes. More specifically, the methods involve introducing the DNA that is to be inserted into the induced break into the plant cell via direct DNA delivery and incubating the plant cells in a plant phenolic compound prior to DNA delivery. Also provided are improved I-SceI encoding nucleotide sequences having a CG content of 50-60%.

Targeted DNA insertion in plants

Methods and means are provided to improve targeted DNA insertion in plants using rare-cleaving 'double stranded break' inducing enzymes. Also provided are improved I-SceI encoding nucleotide sequences.

Growing community of inventors

Ghent, Belgium

Chantal Vanderstraeten