Centre National De La Recherche Scientifique

Université D'orleans

Université D'evry-Val-D'essonne

Chantal Pichon

Patrick Midoux

Michaël Delalande

Mathieu Sassatelli

Matthias Lebertre

Philippe Guegan

Hervé Cheradame

Jean-Marc Malinge

Thomas Thibault

Anthony Delalande

The invention describes a device () for dispensing microbubbles () for cell sonoporation, comprising:

Device for dispensing microbubbles for cell sonoporation

A method for the in vitro production of DNA minicircles includes steps of: a) providing nicked double-stranded oligodeoxynucleotides bunt-ended substrates having at least one phosphorylated 5' end, b) performing a ligase-mediated circularization on a reaction mixture including the nicked double-stranded oligodeoxynucleotides substrates and a DNA bending protein, and c) obtaining DNA minicircles.

In vitro production of DNA minicircles comprising less than 250 base pairs

The invention relates to a static copolymer of linear polyethylenimine that comprises two monomer units of the following formulas I and II, in which Ris a hydrogen atom or a C-Calkyl radical, Ris a hydrogen atom, a C-Calkyl, aryl or aralkyl group, wherein the alkyl group is a C-Cone, n is a number between 1 and 99% of the total monomers, m is a number between 1 and 99% of the total monomers, and advantageously m+n=100%. The invention also relates to complexes of this copolymer with a nucleic acid, and to the use thereof in in vivo, in vitro or ex vivo gene transfection. 

Polymer derived from linear polyethylenimine for gene transfer

Growing community of inventors

Saint Denis de l'Hotel, France

Chantal Pichon