Centre National De La Recherche Scientifique

Etablissement Francais Du Sang

Universitè De Montpellier

Universite Claude Bernard – Lyon1

Joliette Coste

Chantal Fournier-Wirth

Université De Montpellier 1

Jean-Jacques Vasseur

François Morvan

Carole Chaix

Albert Meyer

Carole Farre

Jean-François Cantaloube

Myriam Lereau

Julie Mayen

Francois Morvan

Jean-Francois Cantaloube

The present invention relates to a modified oligonucleotide having two or more thiol functions, which can be immobilized on a gold surface or on a grafted surface, in particular a surface comprising at least one carbon-carbon double bond or carbon-carbon triple bond or haloacetamide functions, preferably maleimide or acrylamide functions. The invention also relates to a method for detecting a nucleic acid in a biological sample comprising a step of detecting hybridization between a modified oligonucleotide and a target nucleic acid amplified from the biological sample. The invention relates more particularly to a method for detecting, genotyping or sequencing a pathogenic organism, preferably a virus.

Modified oligonucleotides comprising thiol functions and use thereof for detecting nucleic acids

The present invention relates to a thiol compound suitable for forming a chain of oligomers that can be grafted to an oligonucleotide. The invention also relates to an oligonucleotide grafted by such a compound, thus having one or more thiol functions, suitable for being immobilized on a gold surface or on a grafted surface.

Thiol compounds and the use thereof for the synthesis of modified oligonucleotides

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La Plaine Saint Denis, France

Chantal Fournier-Wirth