Shandong Landsee Genetics Co., Ltd.

Changli Ge

Kui Li

Yulian Mu

Kui Xu

Zhiguo Liu

Julang Li

Long Wang

Qimei Chen

Yang Qiu

Yinghui Wei

Xuehui Cai

Provided is a double-gene knockout vector system, a method for preparing porcine fibroblasts with both CD163 gene and CD13 gene being knocked-out, prepared porcine fibroblasts, and a method for preparing a gene-edited pig with both CD163 gene and CD13 gene being knocked-out. The vector system of the present disclosure comprises a CD163 gene knockout vector and a CD13 gene knockout vector. The CD163 gene knockout vector comprises a gene editing vector backbone and a DNA fragment ligated to the gene editing vector backbone, with a nucleotide sequence of the DNA fragment being shown in any one of SEQ ID NOs: 1-3. The CD13 gene knockout vector comprises a gene editing vector backbone and a DNA fragment ligated to the gene editing vector backbone, a nucleotide sequence of the DNA fragment being shown in any one of SEQ ID NOs: 4-6.

Method for preparing porcine fibroblasts with both CD163 gene and CD13 gene being knocked-out

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Rizhao, China

Changli Ge