Naval Medical University

Changhai Lei

Shi Hu

Wenyan Fu

The present disclosure relates to the technical field of biomedicine, and provides an anti-tetrodotoxin humanized antibody Y126C and use thereof. The humanized antibody has a heavy-chain variable region and a light-chain variable region with amino acid sequences shown in SEQ ID NO: 1 to SEQ ID NO: 2, respectively. Affinity analysis shows that the antibody of the present disclosure has prominent affinity. It is proved by experiments that, after mice in an antibody protection group pre-injected with the antibody of the present disclosure are injected with tetrodotoxin, most of mice do not show toxic symptoms, and during continuous observation for one month, no toxic lethality occurs, indicating that the antibody of the present disclosure shows excellent anti-tetrodotoxin effects, excellent preventive or therapeutic effects on puffer fish-related biological injuries, and promising clinical application prospects.

Anti-tetrodotoxin antibody Y126C, and preparation method and use thereof

The present disclosure relates to the technical field of biomedicine, and provides an anti-physaliatoxin single-domain antibody, and a preparation method and use thereof. The single-domain antibody is a VHH antibody with an amino acid sequence shown in SEQ ID NO. 1. Affinity analysis shows that the single-domain antibody of the present disclosure has prominent affinity. It is proved by small animal experiments that, after mice in an antibody protection group pre-injected with the single-domain antibody of the present disclosure are injected with physaliatoxin, no mice shows toxic symptoms, and during continuous observation for one month, no toxic lethality occurs, indicating that the single-domain antibody of the present disclosure shows excellent anti-physaliatoxin effects, excellent preventive or therapeutic effects on jellyfish stings, and promising clinical application prospects.

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Shanghai, China

Changhai Lei