University of Pennsylvania

New York University

Changchun Liu

Haim H Bau

Michael G Mauk

Xianbo Qiu

William R Abrams

Eran Geva

Jinzhao Song

Robert Hart

Daniel Malamud

Frederic Bushman

Scott Sherrill-Mix

Daneil Malamud

Karen E Ocwieja

Xianbo Ceiu

Provided herein are devices for monitoring nucleic acid amplification, nucleic acid amplification monitors, methods of quantifying nucleic acid amplification, methods of identifying an unknown nucleic acid molecule, and systems for monitoring nucleic acid amplification. The disclosed devices, methods, and systems comprise at least one nuclemeter, comprising at least one sample chamber and at least one reaction-diffusion conduit in fluid communication with the chamber.

Devices and methods for monitoring and quantifying nucleic acid amplification

Disclosed herein are methods for multiple stage isothermal amplification of nucleic acid comprising a first substantially isothermal amplification reaction on the nucleic acid to generate a first amplification product and at least one substantially isothermal amplification reaction on the first amplification product to generate at least one second amplification product in an amount sufficient for recovery, testing, or characterization.

Multiple stage isothermal enzymatic amplification

Provided herein include compositions comprising a primer having the nucleic acid sequence of ACeIN-F3_c, a primer having the nucleic acid sequence of ACeIN-B3_a, a primer having the nucleic acid sequence of ACeIN-B3_b, a primer having the nucleic acid sequence of ACeIN-FIP_e, a primer having the nucleic acid sequence of ACeIN-FIP_f, a primer having the nucleic acid sequence of ACeIN-BIP (or ACeIN-BIP-song), a primer having the nucleic acid sequence of ACeIN-LF; and a primer having the nucleic acid sequence of ACeIN-LB. Also provided are methods of detecting human immunodeficiency virus (HIV) nucleic acids in a sample comprising performing reverse transcription-based loop mediated isothermal amplification (RT-LAMP) on a sample using the previously disclosed compositions.

Compositions, kits, and methods to detect HIV virus

The present disclosure provides devices and methods using separating a fluid&#x2014;e.g., plasma or serum&#x2014;from whole blood. In some embodiments, the devices and methods use hydrophobic or superhydrophobic surfaces to encourage whole blood to contact a selective membrane that extracts the desired fluid component from the blood.

Fluid separator for point of care molecular diagnostics

A plasma separator includes a vertical chamber with a membrane or membranes partially or fully covering the internal chamber walls and forming an inner cavity to accommodate whole blood. The plasma separator further includes a plasma collection space separated from the inner cavity with one or more membranes and a plasma removal port.

Plasma separator apparatus and associated methods

Provided are devices adapted to isolate, amplify, and detect nucleic acids that may be present in a biological sample. The devices can, in some embodiments, isolate, amplify, and detect nucleic acid in a single chamber. In other embodiments, the devices are adapted to isolate and amplify nucleic acids in a reaction chamber, after which the nucleic acids may be communicated to a pervious medium&#x2014;such as a lateral flow strip&#x2014;where the user may label and detect the nucleic acids.

Isothermal nucleic acid amplification reactor with integrated solid state membrane

Provided are disposable, moisture-activated, self-heating cartridges useful for, e.g., isothermal nucleic acid amplification, incubation, and thermal actuation, and visual fluorescent detection of the amplification products. These devices may be self-contained and do not require any special instruments to operate.

Moisture-activated self-heating analysis device

Provided are analysis cassettes that include removable sealing layers and deformable connector layers that place various channels, chambers, and reservoirs on the cassettes into fluid communication with one another. The cassettes are suitable for use in self-contained, immunoassay devices that may be operated in an automated fashion. Also disclosed are methods for analyzing samples by use of the disclosed cassettes.

Growing community of inventors

Bala Cynwyd, PA, United States of America

Changchun Liu