Nanoentek, Inc.

Chang Seop Lee

Chan Il Chung

Jun Keun Chang

Jun Ha Park

Hyun Chang Lim

Ji Young Park

Hyun Joo Jung

Jeoung Ku Hwang

Jong Sik Jeong

The present invention provides a method for detecting an antigen in an analysis sample, the method including: (a) contacting an analysis sample with a detection antibody with which a marker generating a detectable signal is combined and which specifically binds to the antigen; (b) contacting a capture antibody with the resultant product of step (a), the capture antibody specifically binding to an antigen to be detected; (c) contacting the detection antibody, with which the marker generating a detectable signal is combined, with a reference substance which is bound to a surface of a solid substrate and which includes an epitope to which the detection antibody specifically binds; (d) measuring signals generated from the markers of the resultant product of step (b) and the resultant product of step (c); and (e) analyzing the measured signals to determine the presence or absence and amount of the antigen in the analysis sample. The method for detecting an antigen of the present invention can control the flow and the reaction time of an analysis sample, thereby improving sensitivity and minimizing the influences by the concentration of the analysis sample or the temperature of the detection reaction, and thus improving stability, reliability, and reproducibility of data, when compared with the conventional method for detecting an antigen. Accordingly, the method and apparatus for detecting an antigen of the present invention can be easily operated without specialized skills, thereby instantly obtaining the presence or absence and amount of detection antigen in the analysis sample through on-site diagnosis.

Method for detecting antigen, and apparatus using same

Disclosed is a microfluidic chip capable of accurately and quickly detecting presence of a trace amount of a target within a fluid sample. It has a channel structure comprising a sample inlet for feeding a sample therethrough, a first reservoir for primarily storing the sample therein, a second reservoir for secondarily storing the sample therein, a first reaction portion in which a target is conjugated with a label, a second reaction portion in which the labeled target undergoes a specific reaction, such as an antigen-antibody reaction, and a delaying portion, located between the first and the second reaction portion, for decreasing a flow rate of the sample.

Microfluidic chip for analysis for fluid sample

Disclosed is a module for rapidly detecting analytes in fluids with high effectiveness and a chip having the module. The module includes a microchannel, which has a filtering zone for removing noise materials and a reaction zone wherein labeling reaction and immobilization reaction for detection of analytes are performed, sample fluid moving through the microchannel due to capillary floating. In a case where the chip having the module is used in detecting analytes in fluids, it is possible to minimize dead volume of sample fluid so that high effective volume ratio can be implemented. Therefore, the chip can be used in detecting analytes from the minimum amount of sample fluid.

Module for detecting analytes in fluids and chip having the same

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Ansan-si, South Korea

Chang Seop Lee