Dh Technologies Development Pte. Ltd.

Labcyte Inc.

Other

State Grid Corporation of China

University of Toronto

Hospira, Inc.

State Grid Tianjin Electric Power Company

Xj Group Corporation

Chang Liu

Thomas R Covey

Don Wesley Arnold

John Lawrence Campbell

Yves LeBlanc

Sammy S Datwani

Lucien P Ghislain

David Michael Cox

Thomas Covey

Stephen Alexander Tate

Janusz Boleslaw Pawliszyn

Gary J Van Berkel

Subhasish Purkayastha

Bradley B Schneider

Aaron Ray Wheeler

Gordana Ivosev

Tao Liu

Peter Kovarik

Yan Zhang

Bo Li

German Augusto Gomez Rios

Ke Xu

Stanislaw Potyrala

Yang Kang

Jiqing Mu

Nan Wang

Weijie Wang

Mingchao Yong

Shuibin Zhou

Guanghui Lu

Alexander Tsipirovich

Chao Sun

Xiaohong Kong

Alex Tsipirovich

Bogdan Morosan

Shiqing Feng

See Hai Ng

YiFei Wang

Xue Yao

Zheng Wang

Lingxu Guo

Weibin Song

Wei Liang

Wumin Liang

Chenxi Zhao

Huiquan Duan

Kihwan Choi

Baoyou Fan

Jian Hao

Yishi Zhuang

Shenghui Wang

Hongwei He

Qiuhua Shao

Sheng Lu

Guoyu Wang

Dongwu Gong

Jianyong Liu

Donald W Arnold

A trace of intensity versus time values is received for a series of samples produced by a mass spectrometer. Also, a series of ejections times corresponding to the series of samples produced by a sample introduction system is received. A series of expected peak times corresponding to the series of ejection times are calculated using a known delay time from ejection to mass analysis. At least one isolated peak of the trace is identified using the series of expected peak times. A peak profile is calculated by fitting a mixture of at least two different distribution functions to the at least one isolated peak. For at least one time of the series of expected peak times, an area of a peak at the one time is calculated by fitting the peak profile to the trace at the one time and calculating an area of the fitted peak profile.

Method for increased throughput

An optimal value is calculated for at least one parameter of an ADE device, an OPI, or an ion source device. For each value of a plurality of parameter values for at least one parameter of the ADE device, the OPI, or the ion source device, three steps are performed using a processor. First, the at least one parameter is set to the value. Second, the ADE device, the OPI, the ion source device, and a mass spectrometer are instructed to produce one or more intensity versus time mass peaks for a sample. Third, a feature value is calculated for at least one feature of the one or more intensity versus time mass peaks. A plurality of feature values corresponding to the plurality of parameter values is produced. An optimal value is calculated for the at least one parameter from the plurality of feature values.

AEMS auto tuning

An ADE device identifies an identifiable sequence of one or more ejections from at least one sample using a different value or pattern of values for one or more ADE parameters. The identifiable one or more ejections are performed to produce one or more mass peaks that have a different feature value or pattern of feature values for one or more peak features than other mass peaks produced. Ejection times are stored. One or more detected peaks with the different feature values or pattern of feature values are identified as produced by the identifiable one or more ejections. A delay time is calculated from the time of the identifiable ejections and the time of the identified detected peaks and the peaks are aligned with samples using delay time, stored times, and order of the samples.

Identification of a first sample in a series of sequential samples

Methods and apparatus for processing fluids are described. In various aspects, a fluid processing system may include a magnetic assembly that includes a plurality of magnetic structures configured to generate a magnetic field gradient within a fluid container. The magnetic structures may be formed as a plurality of electromagnets configured to be individually actuated by a controller. Each of the electromagnets may generate a magnetic field within the fluid container. The electromagnets may be differentially actuated to create a magnetic field gradient within the fluid container to agitate, mix, or otherwise influence magnetic particles disposed within the fluid container. Activation of the electromagnets of an electromagnetic structure may generate a magnetic field gradient that influences magnetic particles in an x-y direction. In addition, activation of the electromagnets of a plurality of electromagnetic structures may generate magnetic field gradients that influences magnetic particles in an x-y direction and z-direction.

Electromagnetic assemblies for processing fluids

Each sample of a series of samples is ejected at an ejection time and according to a sample order. Each ejected sample of the series is ionized, producing ion beam. A list of different sets of MRM transitions is received. Each set of the list corresponds to a different sample. A group of one or more different sets is selected from the list. Initially, each set selected for the group corresponds to a different sample of one or more first samples of the series. A mass spectrometer is instructed to execute each transition of each set of the group on the ion beam until a transition of a set of the group is detected, upon which, one or more next sets are selected from the list to be monitored using the set of the detected transition and the sample order.

Simplification of method or system using scout MRM

MS-based methods and systems are provided herein in which a desorption solvent desorbs one or more analyte species from an SPME device within a sampling interface that is fluidly coupled to an ion source for subsequent mass spectrometric analysis. In accordance with various aspects of the applicants teachings, the sampling interface includes an internal sampling conduit that provides increased interaction between the desorption solvent and the sampling substrate, thereby improving mass transfer (e.g., increased extraction or desorption speed).

Sampling probe with internal sampling for use in mass spectrometry systems and methods

A fluid processing system that can include a sample container having a sample chamber for containing a fluid and a plurality of magnetic particles and at least one movable magnetic assembly configured to be movably inserted into or out of the sample chamber. The movable magnetic assembly can include a plurality of electromagnets that generate a magnetic field within at least a portion of the sample chamber when the assembly is inserted at least partially into the sample chamber. The fluid processing system can also include a signal generator that applies electrical signals, e.g., AC electrical signals, to the electromagnets of the magnetic assembly and a controller coupled to the signal generator that is configured to control phases of the electrical signals applied to the electromagnets to generate magnetic field gradients within the portion of the sample chamber effective to magnetically influence the plurality of the magnetic particles.

3-d mixing and particle delivery via movable electromagnets assemblies

Electromagnetic systems and corresponding methods for assembling the electromagnetic systems are described. The electromagnetic systems can be used in fluid processing systems that include a plurality of fluid containers, each configured to define a fluid chamber that receives a fluid and a plurality of magnetic particles, and a plurality of electromagnets configured to generate a magnetic field within at least one of the plurality of the fluid containers. The fluid processing system can also include a PCB board that supplies the electromagnets with electrical current by establishing an electrical connection between electrical contact terminals included on the PCB board and spring loaded connections included on each electromagnet. A control component controls the electromagnetic field generated by each electromagnet to generate a plurality of magnetic field gradients within the at least one fluid container sufficient to magnetically influence the plurality of magnetic particles within the fluid in each fluid container.

Electromagnetic coil assembly structure for processing fluids and methods for making same

Improvements in acoustically dispensed samples that are injected into an open port probe (OPP) are described. Apparatus and method are described that calibrate the volume dispensing determination and mechanisms in the acoustic dispenser to produce accurate and precise volumetric delivery.

Volumetric measurement of micro droplets

A method for the repeated analysis of a sample bearing location. The sample bearing location may include, for instance, a sampled point in a tissue slice that is spatially and temporally correlated to the original slice. The slice may be in whole, or in part, a complete item or a portion of a complete item such as, for example, a human organ. The method improves the analysis process, such as mass spectrometry analysis, by providing a much more complete characterization of the target. The method also allows for the splitting of the sample and chemical/physical alteration of the aliquots for enhanced chemical analysis.

System and method to conduct correlated chemical mapping

Methods and systems for delivering a liquid sample to an ion source for the generation of ions and subsequent analysis by mass spectrometry are provided herein. In accordance with various aspects of the present teachings, MS-based systems and methods are provided in which the flow of desorption solvent within a sampling probe fluidly coupled to an ion source can be selectively controlled such that one or more analyte species can be desorbed from a sample substrate inserted within the sampling probe within a decreased volume of desorption solvent for subsequently delivery to the ion source. In various aspects, sensitivity can be increased due to higher desorption efficiency (e.g., due to increased desorption time) and/or decreased dilution of the desorbed analytes. The methods and systems described herein can additionally or alternatively provide for the selective control of the flow rate of the desorption solvent within the sampling interface so as to enable additional processing steps to occur within the sampling probe (e.g., multiple samplings, reactions).

Methods and systems for increasing sensitivity of direct sampling interfaces for mass spectrometric analysis

A system and method are provided for loading a sample into an analytical instrument using acoustic droplet ejection ('ADE') in combination with a continuous flow sampling probe. An acoustic droplet ejector is used to eject small droplets of a fluid sample containing an analyte into the sampling tip of a continuous flow sampling probe, where the acoustically ejected droplet combines with a continuous, circulating flow stream of solvent within the flow probe. Fluid circulation within the probe transports the sample through a sample transport capillary to an outlet that directs the analyte away from the probe to an analytical instrument, e.g., a device that detects the presence, concentration quantity, and/or identity of the analyte. When the analytical instrument is a mass spectrometer or other type of device requiring the analyte to be in ionized form, the exiting droplets pass through an ionization region, e.g., an electrospray ion source, prior to entering the mass spectrometer or other analytical instrument. The method employs active flow control and enables real-time kinetic measurements.

System and method for the acoustic loading of an analytical instrument using a continuous flow sampling probe

Sampling probes and interfaces for mass spectrometry systems and methods are described to analyze a composition of a substance. The system includes a liquid reservoir containing solvent; a gas reservoir containing nebulizer gas; a conduit that is in fluid communication with the liquid reservoir, the conduit comprising a first and second portion and a junction portion, the first and second portion being joined at the junction portion, and defining an angle therebetween at the junction and the junction portion contains an aperture that is open to the atmosphere. A nebulizer conduit that is co-axial and partially surrounds the second portion and completely surrounds an end of the second portion can also be present, the nebulizer conduit being fluidly connected to the gas reservoir and that allows a gas to flow from the gas reservoir over an external surface of the second portion and the end of the second portion.

Sampling interface for a mass spectrometer

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Richmond Hill, Canada

Chang Liu