Academia Sinica

Industrial Technology Research Institute

National Science Council

Chang-Jen Huang

Hui-Ping Tsai

Po-Ting Chen

Hung-Chi Chien

Yi-Cheng Cheng

Tien-Soung Tong

Shu-Jiau Chiou

Ting-Shou Chen

Yung-Feng Liao

Rita P-y Chen

Cheng-Li Fang

Chih-Ming Chou

Bo-Kai Wu

Chu-Fang Lo

Chung-Hsiung Wang

I-Ching Lu

Gen-Der Chen

Chioh-Yueh Chen

Guang-Hsung Kou

Rita P-Y Chen

Disclosed herein is the novel use of use of aspp. extract, which may upregulate neprilysin, induce autophagy, protect neuron from amyloidopathy or tauopathy, and/or promote neurite outgrowth, thus thespp. extract of the present disclosure may be used as a dietary supplement for the prophylaxis or treatment of amyloid related neurodegenerative diseases so as to ameliorate or alleviate symptoms associated with the amyloid related neurodegenerative diseases.

extract and uses thereof

A genetically modified zebrafish embryo, comprising a HBNF cDNA or a NGF cDNA or a GDNF cDNA together with a cDNA encoding green fluorescent protein (GFP) so as to induce neurite outgrowth during said zebrafish embryonic development, and a method of using such genetically modified zebrafish embryo to identify a therapeutic agent are disclosed.

In vivo assay for neurite outgrowth in zebrafish and its application in drug screening

Two pairs of PCR primer mixtures to be used to detect baculovirus infection: (i) a mixture of 25 to 35-base oligonucleotides including, respectively, 5'-TTTTGACGCAAATYYTAGACGCCGT-3' (SEQ ID NO: 1), and a mixture of 19 to 29-base oligonucleotides, including, respectively, 5'-TCARYATKGATTGAATWTC-3' (SEQ ID NO: 2); and (ii) a mixture of 30 to 40-base oligonucleotides, including, respectively, 5'-TAYGTGTACGACAACAARTAY-3' (SEQ ID NO: 3), and a mixture of 30 to 40-base oligonucleotides, including, respectively, 5'-GCGTCKGGYGCAAAYTCYTTWACY-3' (SEQ ID NO: 7).

Oligonucleotides for detection of baculovirus infection

Growing community of inventors

Taipei, Taiwan

Chang-Jen Huang