Ultima Genomics, Inc.

Labrador Diagnostics LLC

Theranos, Inc.

Chandan Shee

Gilad Almogy

Elizabeth A Holmes

Nathan Beckett

Clarissa Lui

Phillip You Fai Lee

Florian Oberstrass

Joseph Anthony

Jose Martin Sosa

Jerald Sapida

Jerzy Majka

Katrina Sullivan-Bibee

Ushati Das Chakravarty

Kristine Salazar

Bernardo Araujo

Abizar Lakdawalla

Omer Barad

Yuto Watanabe

The present disclosure provides methods and processes for increasing the efficiency and accuracy of nucleic acid sequencing using techniques such as polymerase chain reaction (PCR). Methods and systems provided herein may facilitate performing reactions such as emulsion PCR (ePCR) on samples comprising nucleic acids and beads. The methods provided herein may provide a higher throughput compared to existing technologies.

Methods and systems for nucleic acid analysis

The present disclosure provides methods and systems for processing nucleic acid samples. Methods for processing a nucleic acid sample may comprise providing a double-stranded nucleic acid molecule comprising a partially denaturable region; partially denaturing the partially denaturable region of the double-stranded nucleic acid molecule, thereby generating a region comprising two single strands; and hybridizing a priming sequence to a sequence of one of the single strands. The methods described herein may facilitate amplification without the need for a multitude of complex steps or numerous reagents.

Nucleic acid clonal amplification and sequencing methods, systems, and kits

The present disclosure provides methods and processes for increasing the efficiency and accuracy of nucleic acid sequencing using techniques such as polymerase chain reaction (PCR). The methods described herein can be used to achieve clonal amplification even with a greater than Poisson distribution of beads and/or nucleic acid templates into an emulsion. A PCR method may comprise generating a partition (e.g., a droplet) comprising at least two beads and/or at least two nucleic acid molecules and generating clonal amplification products corresponding to the nucleic acid molecule, at least a subset of which may be attached to a bead.

Methods for nucleic acid analysis

Systems and methods for determining pathogens and antimicrobial resistance of pathogens in a sample are provided.

Pathogen and antimicrobial resistance testing

Applicant discloses herein kits for identifying the presence of Zika virus in a sample. In embodiments, these kits comprise reagents disclosed herein. Applicant further provides kits for use in detecting ZIKV in a sample, the kits comprising reagents disclosed herein. In embodiments, kits include primers directed to Zika virus (ZIKV) nucleic acid sequences, the primers capable of hybridizing to ZIKV nucleic acids and to copies of ZIKV nucleic acids (including to cDNA copies of ZIKV nucleic acids). Applicant discloses herein reagents for detecting Zika virus (ZIKV) in a sample, the reagents including one or more nucleic acid primers that are capable of hybridizing to a ZIKV nucleic acid (including to cDNA copies of ZIKV nucleic acids).

Methods and compositions for detection of Zika viral infections

Growing community of inventors

Newark, CA, United States of America

Chandan Shee