Rutgers, the State University of New Jersey

Bar-ilan University

Chaim Frenkel

Chee-Kok Chin

Daphna Havkin-Frenkel

Bezalel Kessler

Abraham Kessler

Daphna Frenkel

A method for culturing fungal and plant cells in a culture medium containing an elicitor to increase production of a metabolite produced by the cells. For example, tobacco leaves produce the metabolite nicotine and the nicotine is produced in significantly greater amounts when the tobacco is cultured in a elicitor-containing medium. The elicitor is an oxidized Bovine Serum Albumin (BSA), or a glycosylated BSA, or an oxidized lysozyme, or glycosylated lysozyme or azetidine-2-carboxylic acid. Further, metabolite production is increased 1000% over the production of the metabolite when an elicitor is not added during the culturing method.

Method of using an elicitor to increase production of metabolites in

The invention provides a process for stimulating the multiplication, differentiation or somatic embryogenesis of higher plant cells in tissue culture by the addition to the culture medium an effective amount of extensin preparation derived from higher plant tissue. Also, provided are tissue cultures which have such stimulating amount of extensin present. Finally, provided by the invention is the sterile extensin preparation which has the stimulating activity, preferably the extensin used being pure or substantially pure.

Method for stimulating cell multiplication, differentiation,

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North Brunswick, NJ, United States of America

Chaim Frenkel