Wisconsin Alumni Research Foundation

University of Minnesota

Chad L Myers

Justin Theodore Nelson

Jeff Scott Piotrowski

Trey Kyle Sato

Raamesh Deshpande

Scott William Simpkins

Disclosed in some examples are methods including selecting a first plurality of single gene mutants from a pool of possible single gene mutants of an organism. The first plurality of single gene mutants is less than a number of possible single mutants. A computer processor is used to iteratively select a second plurality of single gene mutants by selecting single gene mutants from the pool of possible single gene mutants that increases a sum of products of similarities between the first plurality of single gene mutants and corresponding functional relationships. The second plurality of single gene mutants is larger in number than the first plurality of single gene mutants, and wherein the second plurality of single gene mutants is less than the number of possible single gene mutants of the organism. A set of genes is outputted comprising the first and second pluralities of single gene mutants.

Screening of large-scale genetic interaction networks

The present invention relates to materials and methods for the production of ethanol. More particularly, the present invention provides genetically modified strains ofhaving enhanced tolerance for gamma valerolactone (GVL) toxicity. Also provided are methods of using such genetically engineered yeast strains for improved GVL-mediated hydrolysis of lignocellulosic biomass for industrial-scale ethanol production.

Recombinant yeast having enhanced gamma valerolactone tolerance and methods of use

Growing community of inventors

Arden Hills, MN, United States of America

Chad L Myers