The University of Texas System

Chad E Mire

Thomas W Geisbert

Robert W Cross

Sergio E Rodriguez

Joan B Geisbert

Certain embodiments are directed generally to compositions and methods related to recombinant vesicular stomatitis virus vectors (&#x394;GrVSV) encoding Crimean-Congo Hemorrhagic Fever glycoprotein precursor (CCHFV-GPC) and forming a recombinant vesicular stomatitis virus vector encoding Crimean-Congo Hemorrhagic Fever glycoprotein precursor (&#x394;GrVSV-CCHFV-GPC).

Vesicular stomatitis vectors encoding Crimean-Congo hemorrhagic fever antigen

The present invention includes methods of making, and compositions comprising, a uniformly lethal filovirus for outbred small mammals by mutation of the viral genome through serial passages in a small mammal, the method comprising the steps of: obtaining a filovirus strain from a human subject; passing the filovirus strain one or more times by intramuscular injection of one or more filovirus infected tissues into an inbred small mammal until uniform lethality is obtained; passing the filovirus strain in one or more human cell lines; passing the filovirus strain one or more times by intraperitoneal injection of one or more filovirus infected tissues into an outbred small mammal until uniform lethality is obtained; and isolating the uniformly lethal filovirus obtained thereby.

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Galveston, TX, United States of America

Chad E Mire