Northwestern University

Nanosphere, Inc.

Exicure, Inc.

Karlsruher Institut Fur Technologie

University of Texas System

Harvard College

Nanoink, Inc.

Mass. Institute of Technology

Chad A Mirkin

Robert L Letsinger

Robert Elghanian

James J Storhoff

Robert C Mucic

Thomas Andrew Taton

David A Giljohann

Seunghun Hong

Rongchao Jin

C Shad Thaxton

Richard Piner

So-Jung Park

James L Hedrick, Iii

Yunwei Charles Cao

David Alan Walker

Vinayak P Dravid

SonBinh T Nguyen

Yuhuang Wang

Fengwei Huo

Gengfeng Zheng

Dwight S Seferos

Xiaogang Liu

Khalid Salaita

Nathaniel L Rosi

Zijian Zheng

Weston Daniel

Sergei M Gryaznov

Aleksandar Filip Radovic-Moreno

Christopher C Mader

Adam B Braunschweig

Linette Demers

Raja Kannan Mutharasan

Kaylin M McMahon

Keith Andrew Brown

Omar K Farha

Joseph T Hupp

Hua Zhang

Joshua I Cutler

Viswanadham Garimella

Steven Lenhert

George M Whitesides

Milan Mrksich

James J Hickman

John T McDevitt

Nathan C Gianneschi

Mark S Wrighton

Joseph S Fragala

Randall Q Snurr

Raymond Roger Shile

Xiaodong Chen

Yi Zhang

Dimitra Georganopoulou

Xing Liao

David S Ginger

Can Xue

Zhi Li

Ling Huang

Sungho Park

Jwa-Min Nam

Gabriella Metraux

Haixin Lin

Sushant Tripathy

Andrew E Prigodich

Pinal C Patel

Yun-Wei Cao

Pengcheng Chen

Daniel Maspoch

David M Leander

Andrea Luthi

Resham Singh Banga

Natalia Chernyak

Ming Su

Liangliang Hao

William E Briley

Ke Zhang

Gabriella Métraux

Dan Zheng

Colin Michael Calabrese

Lidong Qin

Dwight Seferos

Jinan Chai

Caroline H Ko

Louise R Giam

Janina Luciano

Sarah J Hurst

Samuel A Jensen

Alexander M Spokoyny

Alexander Stegh

Jung-Hyurk Lim

Andrew Lee

Youn-Sang Bae

Peter V Schwartz

Savka Ilieva Stoeva

Rafael Vega

Kacper Skakuj

Dorota Rozkiewicz

George C Schatz

Maria D Cebezas

Robert D Kennedy

Disclosed herein are spherical nucleic acids (SNAs) comprising oligonucleotides comprising one or more modified oligonucleotides, and methods of use thereof. Also disclosed are methods of synthesizing modified oligonucleotides for use in therapeutics, including deoxynucleic guanidine (DNG)-modified oligonucleotides.

Deoxynucleic guanidines (DNG)-modified oligonucleotides and methods of synthesizing deoxynucleic guanidine strands

The present disclosure is directed to spherical nucleic acids (SNAs) comprising a nanoparticle core and an oligonucleotide shell attached to the external surface of the nanoparticle core, wherein the oligonucleotide shell comprises a mixture of class A CpG oligonucleotides and class B CpG oligonucleotides. The disclosure also provides methods of using the SNAs for, e.g., regulation of an immune response and gene regulation.

Sequence multiplicity within spherical nucleic acids

Methods and apparatus comprising a dewetting phase and a polymerization liquid that are immiscible, and can be used for the formation of three-dimensional objects, wherein the method does not require a dead zone. Additionally, methods and apparatus that employ an optically transparent cooling apparatus to mitigate heat generated during the fabrication process, and the use of a mobile phase to provide a shearing interface to reduce interfacial adhesive forces.

Method for the fabrication of three-dimensional objects and apparatus for same

The disclosure is generally related to spherical nucleic acids (SNAs) comprising a protein corona, wherein the SNA comprises (i) a nanoparticle core and (ii) one or more oligonucleotides attached to the surface of the nanoparticle core, wherein the protein corona comprises a plurality of proteins. The disclosure also provides methods of using the same. The disclosure further provides methods of improving stability and/or extending blood circulation half-life of a spherical nucleic acid (SNA), the SNA comprising a nanoparticle core and one or more oligonucleotides attached to the surface of the nanoparticle core, the method comprising adsorbing a plurality of proteins on the surface of the SNA.

Spherical nucleic acids with tailored and active protein coronae

The present disclosure is directed to core-shell nanoparticles, compositions comprising core-shell nanoparticles, and methods of their use.

Protein/oligonucleotide core-shell nanoparticle therapeutics

A method of site-selective growth of a nanocrystal from an anisotropic seed can include immersing an anisotropic seed functionalized with a ligand in a growth solution having a nanocrystal precursor, a complexing agent, and a reducing agent to form a growth solution, wherein an amount of the reducing agent and/or any amount of the complexing agent is selected to define a supersaturation of the growth solution that is sufficient for overcoming an energy barrier of one or more selected regions of the functionalized seed to selectively growth the nanocrystal at the one or more selected regions.

Method for controlled growth of nanocrystals

Methods and apparatus comprising a dewetting material and a polymerization liquid that are immiscible and dewetting, and can be used for the formation of three-dimensional objects, wherein the method does not require a dead zone. Additionally, methods and apparatus that employ the use of a flowing dewetting material to provide a shearing interface to reduce interfacial adhesive forces.

Rapid, large volume, dead layer-free 3D printing

Methods of stabilizing DNA-engineered crystals can include cross-linking the hybridized oligonucleotides. Stabilized crystals can have improved chemical and thermal stability.

Stabilized colloidal crystals and methods of stabilizing colloidal crystals

Liposomes termed as small unilamellar vesicles (SUVs), can be synthesized in the 20-50 nm size range, but encounter challenges such as instability and aggregation leading to inter-particle fusion. This limits their use as a therapeutic delivery agent. Increasing the surface negative charge of SUVs, via the attachment of anionic entities such as DNA/RNA, increases the colloidal stability of these vesicles. Additionally, the dense spherical arrangement and radial orientation of nucleic acids exhibits unique chemical and biological properties, unlike their linear counterparts. These liposomal particles, are non-toxic and though anionic, can efficiently enter cells without the aid of ancillary cationic transfection agents in a non-immunogenic fashion. These exceptional properties allow their use as delivery agents for gene regulation in different therapies and offer an alternative platform to metal core spherical nucleic acids.

Liposomal particles, methods of making same and uses thereof

Disclosed herein is a massively parallel patterning tool for the deposition of single metals or metal alloys with size and composition control. Methods of the disclosure use a hydrogel array of pyramidal pen tips as a medium for localized electrodeposition, in conjunction with a scanning probe lithography platform and a three-electrode cell. This versatile technique can be used for high-throughput 3D printing, biomolecule patterning, or screening of catalyst nanoparticles or thin films.

Electrochemical polymer pen lithography

The present disclosure generally relates to metal-organic framework nanoparticles containing terminal phosphate-modified oligonucleotides, methods for making the same, and methods of using the same.

General and direct method for preparing oligonucleotide-functionalized metal-organic framework nanoparticles

Provided herein are methods of preparing tetrahexahedra nanoparticles and methods of using the tetrahexahedra nanoparticles as an oxidative catalyst.

Tetrahexahedra nanoparticles

The present invention relates to compositions and methods for delivering an oligonucleotide-functionalized nanoparticle.

Delivery of oligonucleotide-functionalized nanoparticles

A post-synthetic method for stabilizing colloidal crystals programmed from nucleic acid is disclosed herein. In some embodiments, the method relies on Agions to stabilize the particle-connecting nucleic acid duplexes within the crystal lattice, essentially transforming them from loosely bound structures to ones with very strong interparticle links. In some embodiments, the nucleic acid is DNA. Such crystals do not dissociate as a function of temperature like normal DNA or DNA-interconnected colloidal crystals, and they can be moved from water to organic media or the solid state, and stay intact. The Ag-stabilization of the nucleic acid (e.g., DNA) bonds is accompanied by a nondestructive contraction of the lattice, and both the stabilization and contraction are reversible with the chemical extraction of the Agions, e.g., by AgCl precipitation with NaCl.

Stabilization of colloidal crystals engineered with nucleic acid

A method of preparing a metal nanoparticle can include depositing an ink on a substrate using nanolithography to form a polymer reactor, wherein the ink comprises at least one metalloporphyrin functionalized polymer comprising a metal ion, porphyrin, and a polyethylene oxide polymer; and thermally annealing the polymer nanoreactor under conditions sufficient to form the nanoparticle. The thermal annealing can include a first stage that includes annealing the polymer nanoreactor at a temperature of about 100&#xb0; C. to about 350&#xb0; C. for a time sufficient to aggregate the metal ions in the polymer reactor, and a second stage that includes annealing the aggregated polymer nanoreactor at a temperature of about 500&#xb0; C. to about 600&#xb0; C. for a time sufficient to reduce the metal ion and decompose a polymer component of the polymer nanoreactor to thereby form the metal nanoparticle.

Polymer-assisted synthesis of nanoparticles

Growing community of inventors

Wilmette, IL, United States of America

Chad A Mirkin