Commissariat &#xc0; L'&#xc9;nergie Atomique Et Aux &#xc9;nergies Alternatives

Horiba Abx Sas

Iprasense Sas

Illumina France Sarl

Cédric Allier

Salim Boutami

Patrick Pouteau

Lionel Herve

Cyril Delattre

François Templier

Myriam-Laure Cubizolles

Arnaud Rival

Mathieu Dupoy

Thomas Bordy

Johanna Spiaczka

Pierre Joly

Jean-Guillaume Coutard

Eric Marechal

Pierre Blandin

Pierre Marcoux

Olivier Cioni

Michel Drancourt

Jérôme Boutet

Vincent Poher

Emmanuelle Schultz

Sébastien Becker

Geoffrey Esteban

Selimen Benahmed

Sophie Morel

Ondrej Mandula

Maxime Huet

Melissa Baque

Jean-Marc Dinten

Srikanth Vinjimore Kesavan

Giovanni Finazzi

Anais Ali Cherif

Samy Strola

Yves Hennequin

Daniel Vellou

Dorothée Krämer

The invention describes an iterative reconstructing method allowing a spatial distribution of fluorescence in an object to be obtained. The method comprises acquiring images of fluorescence in various planes at various depths in the object, so as to form a three-dimensional acquired image. It comprises an iterative reconstructing algorithm with, in each iteration, an initial fluorescence distribution or a fluorescence distribution resulting from a preceding iteration being taken into account, and the fluorescence light wave propagating through the object being simulated, so as to obtain a reconstruction of the acquired image. The acquired image, or a differential image corresponding to a comparison between the acquired image and the reconstructed image, is then back-propagated through the object, so as to update the fluorescence distribution. FIG.B.

Method for estimating a three-dimensional spatial distribution of fluorescence, inside an object

Method for detecting or predicting the occurrence of a cell event, selected from between cell division or cell death, in a sample (), the sample extending in at least one sample plane (P) and comprising cells () immersed in a medium, the method comprising the following steps:

Method for detecting a cell event

A method for characterizing a particle present in a sample, the sample lying between an image sensor and a light source and the sensor lying in a detection plane, includes illuminating the sample with the light source which emits an incident light wave propagating along a propagation axis, and acquiring an image of the sample with the sensor. The sensor is exposed to an exposure light wave. The image includes a plurality of elementary diffraction patterns each corresponding to one particle. The method also includes reconstructing a complex image representative of a complex amplitude of the light wave on a reconstruction surface passing through the sample, based on the acquired image; selecting a region of interest of the complex image corresponding to a particle of interest; forming an extracted image based on the region of interest; and characterizing the particle of interest depending on the extracted region of interest.

Method for characterising a particle on the basis of a hologram

In accordance with embodiments herein, microfluidics systems and methods are described for identifying and/or tracking particles in a droplet. For example, the particle may be a bead, a cell, or any other type of particle. For example, embodiments herein are useful in distinguishing cells from other particles. The microfluidics systems and methods provide the capability to image a large area (e.g., a few square millimeters) within a digital fluidics chamber using interference microscopy, wherein the image of the interference pattern is acquired, instead of an image of the micrometric object itself. The interference pattern results from the incoming light that interferes with the light scattered by the object. In the case of micrometric objects (e.g., cells, bacteria, etc.), the acquired interference pattern may typically be about 100 jum in diameter so that the area can be imaged using a lens-free imaging configuration or using a low magnification lens.

Systems and methods of identifying and/or tracking particles in a droplet, wherein the particle can be a cell

The method is provided for characterizing agglomeration of particles in a liquid containing an analyte, including introducing liquid into a fluid chamber; mixing the liquid with a bifunctional reagent, lighting the fluid chamber using an excitation light beam extending through the fluid chamber in a longitudinal direction (X); acquiring at least one image using a matrix photodetector, each image including pixels (I(x,y), x,y representing the coordinates of a pixel of an image, the image (I(x,y)) being formed by radiation transmitted by the lighted fluid chamber; and calculating from at least one acquired image (I(x,y)), at least one indicator (Ind) characterizing the particle agglomeration. The photodetector can be positioned less than 1 cm from the fluid chamber, and during the calculation step, the calculated indicator (Ind) is representative of the intensity of the pixels of the image. The method advantageously allows for characterizing the agglomeration of particles in a liquid.

Method and system for characterizing the agglomeration or speed of particles contained in a liquid, such as blood particles

A method for reconstructing optical properties of a diffracting object immersed in a liquid medium using a reconstruction system that comprises a spatially coherent light source and a matrix photodetector, wherein the liquid medium and the matrix photodetector are separated by a distance along a vertical direction. The method comprises illuminating the liquid medium, measuring (with the matrix photodetector) an intensity of a diffraction pattern transmitted by the illuminated medium along a vertical direction, and reconstructing the optical properties of the diffracting object at a reconstruction height according to a reconstruction algorithm from the measured intensity, wherein the reconstruction height has a value less than that of the distance between the medium and the matrix photodetector along the vertical direction.

Method and system for reconstructing optical properties of diffracting objects immersed in a liquid medium

A system for detecting optical particles comprises a transparent slide, a composition arranged on the slide, a light source able to light the composition and the slide, and a device for acquiring at least one image of the composition lit by the light source, the transparent slide being positioned between the light source and the acquisition device. The composition comprises water, the particles, a surfactant and a hydrophilic polymer, the particles having a diameter preferably smaller than 10 pm, still more preferably smaller than 1 pm, the surfactant having a concentration preferably at least equal to the critical micellar concentration, the hydrophilic polymer having a boiling temperature higher than that of water.

System and method for optical detection including a composition for the optical detection of particles, comprising water, the particles, a surfactant and a hydrophilic polymer

This method for estimating the quantity of an analyte contained in a liquid includes the following steps:

Method and system for estimating the quantity of an analyte contained in a liquid

The method according to the invention is capable of characterizing a variation in the speed of particles or agglomeration of particles, the particles, such as blood particles, being contained in a liquid (). The characterization method includes the following steps: During the acquisition step, the photodetector () is positioned at a distance (D) smaller than 1 cm from the fluid chamber () in the longitudinal direction (X).

Method and system for characterizing the movement speed of particles contained in a liquid, such as blood particles

An optical detection process relates to detecting micron- or submicron-sized particles or organisms by means of a contact imaging device, the particles or organisms being immersed in a liquid droplet and the detection being carried out by means of a matrix of photosensitive cells or photosites. The process includes one detection step or a succession of detection steps carried out while the liquid droplet is evaporating. The process may also include a detection step carried out after the liquid droplet has evaporated. The process allows a three-dimensional distribution of the particles or organisms in the initial unevaporated droplet to be reconstructed.

Optical detection process for detecting micron-sized objects in solution

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Grenoble, France

Cédric Allier