Osaka University

Nippon Zenyaku Kogyo Co., Ltd.

Cevayir Coban

Ken Ishii

Shizuo Akira

Toshihiro Tsukui

Yoshikatsu Igari

Yasumasa Kano

Akina Otsuki

Keiichi Ohata

The instant invention is to provide a method for detecting and measuring malaria infection utilizing the induction by hemozoin (HZ); a method for screening a vaccine for malaria infection and a preventative or therapeutic agent for malaria infection using the method for detecting and measuring; and a means for regulating the induction of innate immunity using the HZ, synthetic HZ, or derivatives thereof as an adjuvant or immunostimulant. Malaria infection is detected and measured of by detecting and measuring HZ-induced, TLR9-mediated, and MyD88-dependent innate immune activity. The detection and measurement of malaria infection can be used to diagnose malaria infection. The method for detecting and measuring is also used for screening a vaccine for malaria infection and a preventative or therapeutic agent for malaria infection. Further, HZ, synthetic HZ, or derivatives thereof are used as an adjuvant or immunostimulant to regulate HZ-induced innate immune induction.

Detection/measurement of malaria infection disease utilizing natural immunity by hemozoin induction, screening of preventative or therapeutic medicine for malaria infection disease, and regulation of natural immunity induction

This invention provides a method of preparing a &#x3b2;-hematin crystal comprising a step of heating, the &#x3b2;-hematin crystal obtained by such method, and a vaccine adjuvant composition containing the &#x3b2;-hematin crystal. The &#x3b2;-hematin crystal has a needle-like morphology, it has an average particle size of 0.6 to 1.2 &#x3bc;m, and it exhibits main peaks characteristics for angles of diffraction (2&#x3b8;) of 7.4&#xb0;, 12.2&#xb0;, 21.6&#xb0;, and 24.1&#xb0; in an X-ray diffraction pattern obtained by powder X-ray diffractometry with Cu&#x2014;K&#x3b1; rays (with each peak including a plus-minus 0.2&#xb0; diffraction angle).

Method for producing beta-hematin crystal comprising step of heating

The present invention provides a vaccine adjuvant composition which is used in combination with an allergen vaccine, infection vaccine or tumor vaccine. The present invention specifically provides a vaccine adjuvant composition comprising hemozoin or &#x3b2;-hematin and being used in combination with an allergen vaccine, infection vaccine or tumor vaccine, and a vaccine composition comprising the vaccine adjuvant composition and an allergen vaccine, infection vaccine or tumor vaccine.

Adjuvant

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Osaka, Japan

Cevayir Coban