Biomerieux, Inc.

Celine Roger-Dalbert

Sylvain Orenga

John Perry

Arthur James

Daniel Monget

Michel Peyret

Marine Casse

The present invention relates to a method for identifying at least two groups of microorganisms expressing the same enzymatic activity, comprising the following steps:

Method for identifying at least two groups of microorganisms

The invention relates to a method for detecting and/or identifying() in a biological sample, that comprises inoculating the biological sample liable to containon a detection medium that comprises tryptophan and a substrate for an enzyme A, expressed by the majority of, in order to obtain bacterial colonies; detecting the colonies expressing the activity of the enzyme A and identifying them as being; and detecting the colonies that do not express the activity of the enzyme A, carrying out an indole test, and identifying the colonies having a positive indole test as being

Medium for detecting and/or identifying bacteria

A detection medium including a substrate for a metabolic activity specific for a group of Gram-negative bacteria, a beta-glucosidase or cellobiosidase substrate, and a beta-glucosidase or cellobiosidase inducer. The inducer is a carbohydrate constituted of a carbohydrate linked in the &#x3b2;-position to glucose, or a carbohydrate with a &#x3b2;glucoside subunit, preferably selected from cellobiose, cellulose, starch, cellotriose, and trehalose.

Detection medium for gram negative bacteria

The invention relates to a medium for detecting and/or identifying microorganisms present in a sample, comprising a culture medium and at least one substrate that can be hydrolysed to a labelled product by at least a first enzyme not free in the sample, and specific for the microorganisms, characterized in that it also comprises at least one inhibitor of at least a second enzyme, different from the first enzyme or identical to it, but free in said sample and not originating from a microorganism. The present invention also finds a preferred application in the field of biomedical diagnosis or in food microbiology, and more particularly in bacteriology and mycology.

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Chazey sur Ain, France

Celine Roger-Dalbert