Université Libre De Bruxelles

Delphi Genetics

Delphi Genetics S.a.

R.p.scherer Technologies, Inc.

Cédric Szpirer

Philippe Gabant

Laurence Van Melderen

Michel C Milinkovitch

Jean-Christophe Renauld

Sophie Scohy

Laurent Gillet

Muriel Lemaire

Benjamin Michel

Laurie Dumoutier

Yannick Nizet

Alain Vanderplasschen

Jonathan Cavrenne

Disclosed is a host cell for producing a recombinant peptide, polypeptide or protein of interest, wherein the host cell includes at least 2 copies of a nucleic acid sequence encoding a poison protein; and to the use thereof for producing peptides, polypeptides or proteins of interest.

Host cell for producing proteins

Methods are disclosed for generating antibodies and an expression vector used to express protein(s) which provoke the antibody response. The expression vector may be useful in generating an antibody directed to an antigen, comprising a gene in operable linkage with a promoter, which gene encodes upon expressing a fusion protein comprising (i) CD134L, a fragment, homologous or orthologues protein thereof as N-terminal moiety of the fusion protein; and (ii) all or part of an antigenic protein as C-terminal moiety of the fusion protein. To generate the antibodies, the vector is injected into a subject animal, which produces a fusion protein, against which antibodies are generated.

Method for producing antibody using 'naked' expression vector expressing type II transmembrane fusion protein

The present invention relates to a genetically modified phage and use thereof in a method for producing a biomolecule of interest.

Genetically modified phage and use thereof

The present invention is related to integrated method and tools to construct recombinant DNA molecules (to be used as DNA vaccine or gene therapy) without requiring the use of antibiotic(s) resistance gene(s) and without requiring the addition of one or more antibiotic(s) to the culture medium of cells submitted to this recombinant DNA method. The present invention allows to obtain the selection of recombinant host cell(s) transformed by a (exogenous) nucleic acid sequence of interest (extra-chromosomal vector containing the insert) and simultaneously stabilization (stable inheritance) of this (exogenous) nucleic acid sequence of interest into the transformed host cell(s) descendants (maintenance of the nucleic acid sequence of interest in the host cells population).

Reversible, parallel and multitask cloning method and kit

The present invention is related to a method for the selection of recombinant clones having integrated a gene of interest and a nucleotide sequence encoding a functional antidote protein to a toxic molecule, wherein said recombinant clones are the ones which survive following their integration into a host cell comprising in its genome a nucleotide sequence encoding said toxic molecule. The present invention is also related to a nucleic acid construct, a vector comprising said nucleic acid construct, a host cell and a cloning and/or sequencing kit for performing said method.

Method for the selection of recombinant clones comprising a sequence encoding an antidote protein to toxic molecule

Method for the selection of recombination clones comprising a sequence encoding an antidote protein to a toxic molecule

The present invention is related to a reversible, parallel and/or multitask cloning method and kit, which improve the cloning of (preferably multiple) genetic element(s) in a nucleic acid construct such as vector or in chromosome of a cell and the rapid and efficient selection of said construct with a correct integration of said genetic element(s) either in vitro or in vivo.

Growing community of inventors

Fleurus, Belgium

Cédric Szpirer