Commissariat &#xc0; L'&#xc9;nergie Atomique Et Aux &#xc9;nergies Alternatives

Iprasense Sas

Horiba Abx Sas

Glaxosmithkline Intellectual Property Development Limited

Cedric Allier

Lionel Herve

Thomas Bordy

Sophie Morel

Mathieu Dupoy

Geoffrey Esteban

Jean-Guillaume Coutard

Pierre Blandin

Olivier Cioni

Sebastien Becker

Anais Ali Cherif

Salim Boutami

Patrick Pouteau

Pierre Joly

Eric Marechal

Myriam-Laure Cubizolles

Pierre Marcoux

Michel Drancourt

Vincent Poher

Emmanuelle Schultz

Anne-Gaelle Bourdat

Damien Isebe

Selimen Benahmed

Jerome Boutet

Ondrej Mandula

Giovanni Finazzi

Srikanth Vinjimore Kesavan

Melissa Baque

Samy Strola

Martin Pisaneschi

Daniel Vellou

Melissa Hill

Johanna Spiaczka

A method for determining a state of a cell, the cell being placed in a sample in contact with a culture medium, includes illuminating the sample with a light source and acquiring an image of the sample with an image sensor, the image sensor lying in a detection plane. From the acquired image, a position of the cell in a plane parallel to the detection plane is located and a refractive index or a relative refractive index of the cell is estimated, the relative refractive index corresponding to a refractive index of the cell relative to the refractive index of the culture medium. From the estimation, an index of interest of the cell is determined. From the index, a state of the cell among predetermined states is classified, the predetermined states including at least one apoptosis state and one living state.

Method for determining the viability of cells

A device for observing a biological sample is provided, including: a light source to emit a light beam at a wavelength between 1 &#x3bc;m and 20 &#x3bc;m; an image sensor including pixels defining a detection plane; a holder to hold the sample between the source and the sensor at a distance from the plane smaller than 1 mm, such that the source is configured to illuminate an area of the sample larger than 1 mm, no image-forming optics are placed between the sample and the sensor, and the sensor is configured to acquire an image corresponding to an area of the sample larger than 1 mmand representative of an absorption of the beam by the sample at the wavelength; and a processor to determine a map of an amount of analyte in the sample, based on the image acquired by the sensor, the analyte absorbing light at the wavelength.

Device for observing a biological sample in the infrared range

A method for observing a sample (), the sample lying in a plane of the sample defining radial coordinates, the method comprising the following steps:

Method for observing a sample

A method for observation of a sample, the sample comprising microorganisms immersed in a nontransparent culture medium, the culture medium being favorable to the development of the microorganisms, and the sample being arranged between a light source and an image sensor, includes illuminating the sample with the light source, the light source emitting light propagating along an axis of propagation; acquiring an image of the sample by the image sensor; and, from the image acquired, characterizing the microorganisms. Light travels along the microorganisms through the culture medium to the sensor.

Method for early observation of colonies of microorganisms

A method for observing a sample, the sample lying in a sample plane defining radial positions, parameters of the sample being defined at each radial position, the method comprising:

Holographic reconstruction method

A method is provided for observing a biological sample between a light source and a pixelated image sensor, the light emitting an incident light beam, which propagates to the sample along a propagation axis and at an emission wavelength, the method including: illuminating the sample with the source; and acquiring an image of the sample with the sensor, no image-forming optic being placed between the sample and the sensor, the sample absorbing some of the beam, such that the acquired image is representative of an absorption of the beam by the sample at the emission wavelength, the source illuminates an area of the sample larger than 1 mm, the image acquired of the sample by the sensor corresponds to an area of sample larger than 1 mm, and pixels of the sensor define a detection plane, the sample being placed at a distance from the plane smaller than 1 mm.

Method for observing a sample in the infrared range

A method for observing a fluorescent sample, the sample comprising a fluorescent agent that emits fluorescence light, in a fluorescence spectral band, when it is illuminated by excitation light, in an excitation spectral band, the method comprising the following steps:

Device and method for observing a fluorescent sample

The invention relates to a method for tracking the amplification of a sequence of nucleotides in a sample (). The sample is placed between a light source () and an image sensor (). Under the effect of amplification reagents, mixed with the sample, a nucleotide sequence, called the target sequence, is replicated iteratively, amplifying the target sequence. The method includes the acquisition of an image representative of the formation of a precipitate in the sample under the effect of the amplification, on the basis of which an image of interest is formed. The application of a statistical indicator to the image of interest allows an indicator of the amplification of the target sequence to be determined.

Optical method for tracking the in-vitro amplification of a nucleotide sequence

A method for determining a parameter of a particle present in a sample, the method comprising the following steps: The particle may associated with a set of parameters, comprising at least a size of the particle and a refractive index of the particle.

Method for determining parameters of a particle

A method for holographic characterization of a particle contained in a sample, based on an image, or hologram, of the sample obtained by an image sensor when the sample is illuminated by a light source. The hologram is the subject of a holographic reconstruction, to obtain a reference complex image, representative of the light wave transmitted by the sample in a reconstruction plane. A holographic propagation operator is applied to the reference complex image, to obtain a plurality of secondary complex images, from which a profile is determined describing the change in an optical feature of the light wave transmuted by the sample along the axis of propagation of the light wave.

Holographic method for characterizing a particle in a sample

The invention relates to a method for observing a sample (), comprising the illumination of the sample using a light source () and the acquisition of an image (Io) of the sample using an image sensor (), the sample being disposed between the image sensor and the light source. Iterative steps are applied to the acquired image (Io), also referred to as a hologram, comprising: a single iterative numerical propagation (h), such as to estimate a complex image (A) of the sample in a reconstruction plane (P) or in a detection plane (P), in which the image sensor extends. The complex image can be used for the characterisation of the sample.

Method for observing a sample, by calculation of a complex image

There is provided a device allowing a sample to be observed in a first mode, by lensless imaging using a first sensor. The first mode allows a first image to be obtained, on the basis of which a region of interest of the sample may be identified. The device then allows, via a relative movement, the region of interest to be analyzed using a more precise second mode and in particular using an optical system coupled to a second sensor.

Device for observing a sample and method for observing a sample

A method for observing a sample includes illuminating the sample with a light source and forming a plurality of images, by an imager, the images representing the light transmitted by the sample in different spectral bands. From each image, a complex amplitude representative of the light wave transmitted by the sample is determined in a determined spectral band. The method further includes backpropagation of each complex amplitude in a plane passing through the sample, determining a weighting function from the back-propagated complex amplitudes, propagating the weighting function in a plane along which the matrix photodetector extends, updating each complex amplitude, in the plane of the sample, according to the weighting function propagated.

The invention relates to a method for observing a fluorescent sample lying in a sample plane, where the sample comprises a fluorescent agent able to emit a fluorescence light wave in a fluorescence spectral band when it is illuminated by an excitation light wave, in an excitation spectral band. The method includes illuminating the sample using a first light source, in a first illumination spectral band in the excitation spectral band, and acquiring a first image of the sample, in the fluorescence spectral band, using an image sensor; and illuminating the sample using a second light source, in a second spectral band, outside of the fluorescence spectral band, and acquiring a second image of the sample, in the second spectral band, using the image sensor. The image sensor is coupled to an optical system such that in the fluorescence spectral band, the object focal plane of the optical system is coincident with the plane of the sample; and in the second spectral band, the object focal plane of the optical system is offset with respect to the plane of the sample.

Device and method for observing a sample with a chromatic optical system

A method for identifying a state of a cell contained in a sample, including: illuminating the sample using a light source by producing an incident light wave propagating toward the sample; then acquiring, using a matrix-array photodetector, an image of the sample, the sample being placed between the light source and the matrix-array photodetector such that the matrix-array photodetector is exposed to a light wave resulting from interference between the incident light wave and a diffraction wave produced by each cell; applying a numerical reconstruction algorithm to the image acquired by the matrix-array photodetector, to estimate a characteristic quantity of the light wave reaching the matrix-array detector, at a plurality of distances from the matrix-array photodetector. The value of the characteristic quantity, or its variation as a function of distance, allows the state of the cell to be determined from among predetermined states.

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Grenoble, France

Cedric Allier