Commissariat a L'energie Atomique

Centre National De La Recherche Scientifique

Université Joseph Fourier

University of Montpellier Ii

Girindus Ag

Universite Joseph Fourier De Grenoble

Commissariat a L'energie Atomique Et Aux Energies Alternatives

Centre National De La Recherche Scientifique-Cnrs

L'université Montpellier Ii

Cécile Dueymes

Marc Fontecave

Jean-Luc Decout

Jean-Louis Imbach

Philippe Peltie

Jean-Jacques Vasseur

François Morvan

Albert Meyer

Marc Cuzin

Fritz Link

Meinolf Lange

Ilaria Adamo

Andreas Schonberger

A solution phase synthesis method for preparing an oligonucleotide, wherein at least some of the reagents are solid supported. The method suitable for large-scale synthesis comprises coupling a protected compound with a nucleotide derivative having a protection group in the presence of a solid supported activator to give an elongated oligonucleotide with a P(III)-internucleotide bond; optionally processing the elongated oligonucleotide by capping by reaction with a solid supported capping agent and/or by oxidizing or sulfurizing by reaction of the oligonucleotide with a solid supported oxidizing or sulfurization reagent; and removing the protection group. The coupling may include reacting a 3'-protected compound of formula: with a nucleotide derivative having a 5&#x2032;-protection group, or reacting a 5&#x2032;-protected compound of formula with a nucleotide derivative having a 3&#x2032;-protection group.

Method for preparing oligonucleotides

The invention concerns a method for analyzing biological targets of DNA or RNA type, which comprises the following steps:

Method and support for biological analysis using oligonucleotides comprising a marker capable of enzymatic activation

This invention relates to an analytical support containing a plurality of oligonucleotides fixed on this support, where each of the nucleotides is marked by a fluorescent compound that presents a variation of fluorescence on the hybridization of each marked oligonucleotide with at complementary oligonucleotide. This support makes it possible to carry out an analysis of biological targets by measuring the variation of fluorescence in order to determine the hybridization of the targets with the oligonucleotides of the support.

Analysis of biological targets using a biochip comprising a fluorescent marker

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Rodez, France

Cécile Dueymes