Vrije Universiteit Brussel

Vrije Universiteit

Cecile Casterman

Raymond Hamers

There are provided methods for screening a library of Vpolypeptides for Vpolypeptides that bind to an antigen including subjecting a library of Vpolypeptides to antigen-affinity selection with the antigen.

Method for screening a library of VHH polypeptides

The invention relates to an immunoglobulin having determined antigen specificity, and having a variable fragment which is derived from a so-called heavy-chain immunoglobulin having two heavy polypeptide chains capable of recognizing and binding one or several antigens and which is further naturally devoid of light chains, wherein the immunoglobulin having determined antigen specificity is devoid of CH1 constant region between the variable region and the hinge region and has within its constant region at least part of a constant region of a human antibody.

Immunoglobulins devoid of light chains

The invention relates to an immunoglobulin having determined antigen specificity, and having a variable fragment which is obtainable from a camelid heavy-chain immunoglobulin having two heavy polypeptide chains capable of recognizing and binding an antigen and which is further devoid of polypeptide light chains, wherein the immunoglobulin having determined antigen specificity is devoid of a CH1 constant region between the variable region and the hinge region and has within its constant region at least part of a constant region of a human antibody.

There is provided an isolated immunoglobulin comprising two heavy polypeptide chains sufficient for the formation of a complete antigen binding site or several antigen binding sites, wherein the immunoglobulin is further devoid of light polypeptide chains.

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Sint-Genesius-Rode, Belgium

Cecile Casterman