Handylab, Inc.

Cecelia Haley

Kalyan Handique

Gene Parunak

Sundaresh N Brahmasandra

Aaron Kehrer

Betty Wu

Nikhil Phadke

John S Althaus

Ted Springer

The present technology provides for a microfluidic substrate configured to carry out PCR on a number of polynucleotide-containing samples in parallel. The substrate can be a single-layer substrate in a microfluidic cartridge. Also provided are a method of making a microfluidic cartridge comprising such a substrate. Still further disclosed are a microfluidic valve suitable for use in isolating a PCR chamber in a microfluidic substrate, and a method of making such a valve.

Microfluidic valve and method of making same

Methods and systems for processing polynucleotides (e.g., DNA) are disclosed. A processing region includes one or more surfaces (e.g., particle surfaces) modified with ligands that retain polynucleotides under a first set of conditions (e.g., temperature and pH) and release the polynucleotides under a second set of conditions (e.g., higher temperature and/or more basic pH). The processing region can be used to, for example, concentrate polynucleotides of a sample and/or separate inhibitors of amplification reactions from the polynucleotides. Microfluidic devices with a processing region are disclosed.

Method for processing polynucleotide-containing samples

System for processing polynucleotide-containing samples

A sample preparation device for reducing a concentration of one or more concomitant components of a sample and/or increasing a concentration of one or more desired sample components is described.

Sample preparation device and method

Growing community of inventors

Canton, MI, United States of America

Cecelia Haley