Promega Corporation

Ce Shi

Lance P Encell

Thomas A Kirkland

Mary P Hall

Keith V Wood

Poncho Meisenheimer

Brock Binkowski

Thomas Machleidt

Matthew B Robers

Melanie Dart

Michael Killoran

Wenhui Zhou

Hui Wang

Joel R Walker

Harry Tetsuo Uyeda

Sergiy Levin

Sergei Saveliev

Min Zhou

Zhiyang Zeng

Robert Kargbo

Valerie Ressler

Jun Zhang

Jean Osterman

Prabin Rai

Brock F Binkowski

Provided herein are surfactant compounds that can be used to aid in preparation of protein samples for analysis, for example by mass spectrometry.

Acid-labile surfactants

Provided herein are systems and methods for characterizing target/ligand engagement. In particular, luciferase-labeled polypeptide targets are used to detect or quantify target/ligand engagement (e.g., within a cell or cell lysate).

Luciferase-based thermal shift assays

Provided herein are enhanced luciferase enzymes for use with thermostable luciferin analogs for bioluminescent assays. In particular, the present disclosure provides compositions, assays, and methods for performing a bioluminescent assay using enhanced, high-activity luciferase enzymes compatible with thermostable luciferins, such as 5,5-disubstituted luciferin analogs.

Luciferase enzymes for use with thermostable luciferins in bioluminescent assays

Provided herein are inhibitor-resistant luciferase mutants, and methods of use thereof. In particular, luciferase mutants are provided that are thermal stable and exhibit improved stability in the presence of luciferin break-down products, such as dehydroluciferin. Further provided are assay systems comprising inhibitor-resistant luciferase mutants and amino acid sequences of the inhibitor-resistant luciferase mutants.

Thermal stable luciferase with improved resistance to inhibition by luciferin break-down products

Disclosed herein are compositions and methods for stabilizing a benzothiazole luciferin analog such as D-luciferin and 6-amino-D-luciferin. The compositions may include the benzothiazole luciferin analog, a thionucleobase compound of formula (I), and a liquid medium, in which the thionucleobase is present in an amount effective to stabilize the luminogenic composition against decomposition. The methods provided herein may stabilize the benzothiazole luciferin analog against decomposition by contacting the benzothiazole luciferin analog with an effective amount of the thionucleobase compound in the presence of a liquid medium. Also provided herein is a kit containing the composition.

Compositions and methods for stabilizing benzothiazole luciferin analogs

Described are luciferin analogues, methods for making the analogues, kits including the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays.

5,5-disubstituted luciferins and their use in luciferase-based assays

Described are luciferin analogs, methods for making the analogs, kits including the analogs, and methods of using the compounds for the detection of luminescence in luciferase-based assays.

Growing community of inventors

San Luis Obispo, CA, United States of America

Ce Shi