Université Libre De Bruxelles

Catherine Yourassowsky

Frank Dubois

Franck Dubois

Karim Zouaoui Boudjeltia

Daniel Ribeiro De Sousa

The present invention is related to an holographic probe device for recording a gabor hologram comprising: a coherent optical fiber bundle comprising a distal end and a proximal end; a recording medium optically coupled to the proximal end of the coherent optical fiber bundle; a light source producing in use a single light beam, illuminating the distal end of the coherent optical fiber bundle and the object to be observed.

Method for recording gabor hologram

The present invention is related to a method for characterizing transparent objects () in a transparent medium (), said transparent objects () presenting an optical focal area () said method comprising the steps of: illuminating a sample comprising the objects () to be characterized by means of a directional light () source, thereby inducing light intensity peaks () at the focal area of said transparent objects; determining at least one characteristic of the light intensity peak () induced by said object to be characterized, determining from said light intensity peak () at least one property of said object.

Optical method for characterizing transparent particles

An Interferometer for off-axis digital holographic microscopy includes a recording plane, and a grating located in a plane optically conjugated with the recording plane. The grating defining a first and a second optical path, the optical path corresponding to different diffraction order.

Off-axis interferometer

A method and device for obtaining a sample with three-dimensional microscopy, in particular a thick biological sample and the fluorescence field emitted by the sample. One embodiment includes obtaining interferometric signals of a specimen, obtaining fluorescence signals emanating from the specimen, recording these signals, and processing these signals so as to reconstruct three-dimensional images of the specimen and of the field of fluorescence emitted by the specimen at a given time. Another embodiment includes a digital holography microscope, a fluorescence excitation source illuminating a specimen, where the microscope and the fluorescence excitation source cooperate to obtain interferometric signals of the specimen and obtain fluorescence signals emanating from the specimen, means for recording the interferometric signals and fluorescence signals, and means for processing the interferometric signals and the fluorescence signals so as to reconstruct three-dimensional images of the specimen and of the field of fluorescence emitted by the specimen at a given time.

Digital holographic microscope

The present invention is related to a compact microscope able to work in digital holography for obtaining high quality 3D images of samples, including fluorescent samples and relatively thick samples such as biological samples, said microscope comprising illumination means () at least partially spatially coherent for illuminating a sample () to be studied and a differential interferometer () for generating interfering beams from said sample () on the sensor () of an electronic imaging device(), said interferometer () comprising namely tilting means () for tilting by a defined angle one the interfering beams (or) relatively to the other, said tilting resulting into a defined shift () of said interfering beam on the sensor of the electronic imaging device (), said shift () being smaller than spatial coherence width of each beam, said microscope being able to be quasi totally preadjusted independently from the samples so that minimum additional adjustments are required for obtaining reliable 3D images of samples.

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Brussels, Belgium

Catherine Yourassowsky