Ventana Medical Systems, Inc.

Catherine Wolf

Anthony L Hartman

Kimberly Christensen

Jennifer Wong

Charles Daniel Lemme

William L Richards

Wayne A Showalter

Brian Howard Kram

Thomas M Grogan

Austin Micheil Ashby

Joseph G Utermohlen

Andrew Ghusson

Noemi Sebastiao

David Bryant

Ethel R Macrea

Lidija Pestic-Dragovich

Hiroaki Nitta

Alexis Pusch

Angela Sattler

Jacques Cavadenti

James Pettay

Keri Hopkins

Thomas Grogan

The present invention introduces a radically different way of accelerating biomolecule conjugates into tissue, and hence towards their targets for purposes of tissue staining. The invention provides for an order of magnitude improvement over the prior art diffusion process used to stain tissue. The invention comprises a method of tissue staining by applying an electric field to a tissue sample in the presence of an electrolyte and biomolecular conjugates of interest suspended in the electrolyte. Typical staining times are reduced to seconds as opposed to 30-120 minutes common in the prior art. The invention is also directed to devices for performing the method.

Apparatus for electrophoretic in situ tissue staining

The present invention provides reagents for use in an automated environment for removing or etching embedding media by exposing a biological sample to be stained in histochemical or cytochemical procedures without the dependence on organic solvents. The reagents comprise components optimized to facilitate removal or etching of the embedding media from the biological sample. The present invention also provides reagents for use in an automated environment for cell conditioning biological samples wherein the cells are predisposed for access by reagent molecules for histochemical and cytochemical staining procedures. The reagents comprise components optimized to facilitate molecular access to cells and cell constituents within the biological sample.

Removal of embedding media from biological samples and cell conditioning on automated staining instruments

The invention provides novel reagents, reagent kits, and methods for automated hybridization. More particularly, the invention provides reagents, reagent kits, and methods for automated in situ hybridization and automated hybridization on a microarray. The use of automated instruments for in situ hybridization and microarray hybridization dramatically reduces the amount of labor and time involved and also facilitates standardization of protocols and consistency between results.

Reagents and methods for automated hybridization

The invention is directed to an improvement in automated tissue staining system having evaporation inhibitor liquid covering the polynucleotide hybridization buffer-covered tissue on a slide, wherein the improvement comprises a polynucleotide hybridization buffer for in situ hybridization comprising a low molecular weight dextran sulfate having a molecular weight range from about 8,000 to about 16,000.

Hybridization buffers using low molecular weight dextran sulfate and methods for their use

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Eckbolsheim, France

Catherine Wolf