Novo Nordisk A/s

Collaborative Research, Inc.

Catherine M Buckley

Gerald R Fink

Steen Troels Jo Slashedrgensen

David John McConnell

Audrey Hobson

Boerge Krag Diderichsen

A process for producing an active lipase enzyme in vitro, comprising mixing an inactive or partly active lipase enzyme with a chaperone molecule and subjecting the mixture to denaturation followed by renaturation to produce the active lipase enzyme.

Process for preparing lipases

This invention relates to the production of useful protein products by secretion from yeast cells. In particular, it relates to the identification of supersecreting mutant yeast cells, isolation of the wild-type gene which complements the mutation, and construction of non-leaky, non-reverting supersecreting strains.

Supersecreting mutants of saccharomyces cerevisiae

Growing community of inventors

Cork, Ireland

Catherine M Buckley