Institut National De La Sante Et De La Recherche Medicale

Université Paris Diderot-paris 7

Université Paris 7—denis Diderot

Université De Bordeaux II Victor Segalen

Universite Paris Cité

Virsol

Centre National De La Recherche Scientifique

Inserm

Catherine Le Visage

Didier Letourneur

Sidi Mohammed Derkaoui

Jean-Christophe Fricain

Sylvain Catros

Frederic Chaubet

Aude Autissier

Joelle Amedee

Nicole Bru-Magniez

Patrick Couvreur

Elias Fattal

Pascal Breton

Nathalie Luciani

Florence Gazeau

Frédéric Chaubet

Joëlle Amedee

Claire Wilhelm-Hannetel

Delphine Fayol

The present invention relates to a method for preparing a porous scaffold for tissue engineering. It is another object of the present invention to provide a porous scaffold obtainable by the method as above described, and its use for tissue engineering, cell culture and cell delivery. The method of the invention comprises the steps consisting of: a) preparing an alkaline aqueous solution comprising an amount of at least one polysaccharide, an amount of a cross-linking agent and an amount of a porogen agent b) transforming the solution into a hydrogel by placing said solution at a temperature from about 4&#xb0; C. to about 80&#xb0; C. for a sufficient time to allow the cross-linking of said amount of polysaccharide and c) submerging said hydrogel into an aqueous solution d) washing the porous scaffold obtained at step c).

Method for preparing porous scaffold for tissue engineering, cell culture and cell delivery

The present inventions relates to beads as biocompatible material adapted for use within the human or animal body. Said beads are highly useful for tissue engineering, in situ tissue regeneration, as well as for drug and/or cells delivery. In addition, said beads may support biotechnological applications such as cell carriers.

Crosslinked polysaccharide beads and their biomedical uses

The present invention relate to three dimensional porous polysaccharide matrices able to induce mineralization of a tissue in osseous site, as well as in non-osseous site, in the absence of stem cells or growth factors.

Porous polysaccharide scaffold comprising nano-hydroxyapatite and use for bone formation

The present invention relate to three dimensional porous polysaccharide matrices able to induce mineralisation of a tissue in osseous site, as well as in non-osseous site, in the absence of stent cells or growth factors.

The present invention relates to a method for preparing a porous scaffold for tissue engineering. It is another object of the present invention to provide a porous scaffold obtainable by the method as above described, and its use for tissue engineering, cell culture and cell delivery. The method of the invention comprise the steps consisting of a) preparing an alkaline aqueous solution comprising an amount of at least one polysaccharide and one cross-linking agent b) freezing the aqueous solution of step a) c) sublimating the frozen solution of step b) characterized in that step b) is performed before the cross-linking of the polysaccharide occurs in the solution of step a).

Method for preparing porous scaffold for tissue engineering

The present invention relates to a method for preparing a porous scaffold for tissue engineering. It is another object of the present invention to provide a porous scaffold obtainable by the method as above described, and its use for tissue engineering, cell culture and cell delivery. The method of the invention comprise the steps consisting of a) preparing an alkaline aqueous solution comprising an amount of at least one polysaccharide and one cross-linking agent b) freezing the aqueous solution of step a) c) sublimating the frozen solution of step b). characterized in that step b) is performed before the cross-linking of the polysaccharide occurs in the solution of step a).

Poly(methylidene malonate) microspheres, preparation method and pharmaceutical compositions containing them

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Catherine Le Visage